摘要
目的:研究正常人肠道的乳杆菌对常用10种抗生素的耐受情况,为临床选药提供有效的依据,为耐药机制和耐药性传递等方面的研究提供基础数据。方法:采用微量肉汤稀释法(M IC)测定43株正常人肠道乳杆菌对10种抗生素的耐受性。结果:受试菌对万古霉素的耐受性最强(100%),其次是对环丙沙星和左氟沙星的耐受性(99.7%);对庆大霉素、四环素、头孢呋辛和头孢唑啉表现出不同程度的耐受;对利福平较敏感,而对青霉素和氨苄西林非常敏感(100%);所有菌株均耐受2类及以上的抗生素。结论:在试验所用抗生素中,正常人肠道乳杆菌对万古霉素的耐受性最强,对青霉素、氨苄西林和利福平较敏感,受试乳杆菌对同类抗生素的耐受差异小。
Objective：A collection of intestinal lactobacillus from the healthy human were investigated for tolerance to 10 categories of antimicrobial agents,which may be the basis of the choice for clinical treatment. It is also helpful for the following study about the mechanism of resistance and how to transfer genes to the other bacterias supply some information. Methods： With microbroth dilution techniques of MIC, the antibiotic susceptibility of 43 strains in enteric area of the normal human were determined with 10 antibiotics. Results：All isolates strains were tolerant to vancomycin（ 100% ）, and most strains were tolerant to levofloxacin（99.7% ）, Some strains were tolerant to gentamycin,tetracycline, ciprofloxacin, while only a few strains were sensitive to rifampicin. All lactobacilli isolates were sensitive to penicillin and ampiciUin and tolerant to two or more kinds of antibiotics. Conclusion： The results shown that the level of tolerance to vancomycin was the higist. The lactobacillus were susceptible to low concentration of rifampicin, penicillin and ampicillin. At the same time, there was no observable difference of the tolerance between two types of antibiotic.
出处
《中国卫生检验杂志》
CAS
2008年第7期1365-1366,1386,共3页
Chinese Journal of Health Laboratory Technology
关键词
乳杆菌
抗生素
耐受性
Lactobacillus
Tolerance
Antibiotics
