摘要
建立了测定大鼠血浆中硫普罗宁含量的柱前衍生-高效液相色谱荧光法。血浆样品经二硫苏糖醇（DTT）还原后，再经N-（1-芘）马来酰亚胺（NPM）衍生化后进样测定。以0．2％乙酸（含0．015 mol·L^-1磷酸二氢钾）-乙腈（56：44）为流动相，采用Kromasil C18色谱柱分离，在λex=340nm，λem=375nm下荧光检测。硫普罗宁线性范围为0．1～10．0μg·mL^-1，定量下限为0．1mg·L^-1，日内、日间精密度（RSD）分别为5．3％～10．8％和7．0％～10．8％，提取回收率在73．7％～79．7％。大鼠单剂量给予硫普罗宁后，药代动力学行为符合二室模型。该法准确可靠，专属性强，适用于硫普罗宁的药代动力学研究。
A sensitive, rapid method for determining reduced tiopronin concentration in rat plasma has been developed by using a high-performance liquid chromatography （HPLC） technique in conjunction with the derivatizing agent N-（1-pyrenyl）maleimide （NPM）. The analytes were separated on a Kromasil C）s column （250 mm ×4.6 mm, 5 μm） using 0. 2% glacial acetic acid aqueous solution including 0. 015 mol · L^-1 KH2PO4 and acetonitrile （56：44） as a mobile phase at a flow-rate of 0. 8 mL · min^-1, and fluorescence detection wavelength were set at λex = 340 nm and λem = 375 nm, the column temperature was 30 ℃. The calibration curve was found to be linear over a range of 0. 1 - 10. 0 μg · mL^- 1 , the limit of quantitation was 0. 1 mg · L^-1. The coefficients of the variation for the within-run and between-run precisions ranged from 5.3% to 10. 8% and 7.0% to 10. 8% , respectively. The percentage of absolute recovery ranged from 73.7% to 79.7%. The method was used to determine the concentration of tiopronin in rat plasma after a single intragastric administration of 25 mg · kg^- 1 tiopronin to 6 healthy male Wistar rats. The pharmacokinetic process was fitted to a two-compartment model. The method has been successfully applied to the determination of tiopronin in rat plasma.
出处
《药学学报》
CAS
CSCD
北大核心
2008年第7期733-736,共4页
Acta Pharmaceutica Sinica
