摘要
红色毛癣菌菌落产生红色是该菌重要鉴定特征之一，但常遇到不产生红色的菌株。了解这种产色变异的范围和频率以及影响因素，对提高鉴定正确性、更好地指导治疗有重要意义。方法：对２００株不同地区来源的临床株进行初代分离和传代培养，并观察１％葡萄糖玉米粉吐温琼脂和马铃薯葡萄糖琼脂对产色的恢复作用；比较不同培养条件对产色变异的影响，对有关数据进行统计学处理。结果：初代产色消失率为３４％，传代诱导消失率为４９％，稳定消失率达２４％。大多数传代消失为可逆的，而大多数初代变异为不可逆的；较高的ｐＨ和较低的培养温度不利于产色。北方分离株产色消失率显著高于南方分离株。结论：红色毛癣菌产色消失率发生较高，已对其鉴定产生影响，其确切的发生机理。
Purpose: The formation of red color on the colony of T.rubrum is an important character for the identification of the fungus. But, those strains which cannot yield red color on the colony are isolated frequently. It is important to investigate the range,frequency and influence factors of the color variation of T.rubrum for the identification of the fungus and directing therapy.Method: 200 strains of T.rubrum from different areas of China were isolated as the first generation and cultured serially. The resumptive actions of culture media (DCTA and PDA) on yielding color were observed,and the influences of different culture conditions on the color variation were compared.Result:The color variation rate of the first generation was 34% in 200 strains tested. The color variation rate induced by serial cultures was 49%. The majority variations induced by serial cultures were reversible, but the majority variations of the first generation were irreversible.Higher pH value of the cultures and lower cultural temperature were unfavorable in the formation of red color. The color variation rate of isolates from the North China was markedly higher than those from the South China. Conclusion:The frequency of color variation of T.rubrum is high that influences the identification of the fungus. The definite mechanism of the color variation remains to be studied.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1997年第5期312-314,共3页
Chinese Journal of Dermatology
关键词
红色毛癣菌
产色消失
变异
Trichophyton rubrum Phenotype Variation
