摘要
利用前导肽序列设计引物，采用ＲＴ－ＰＣＲ技术从分泌抗人胃癌的单抗杂交瘤细胞系３Ｈ１１分离克隆了抗体的轻重链可变区基因序列。经ＤＮＡ序列分析表明所获基因含有全部前导序列，其成熟蛋白编码部分与从第一骨架区引物所克隆的序列相符。将轻链基因组建到人－鼠嵌合轻链表达载体中，转染至小鼠骨髓瘤细胞Ｓｐ２／０中，可获得人鼠嵌合轻链的表达，证明所克隆的基因具有表达活性，为人－鼠嵌合抗体的构建奠定了基础。
The entire V H and V L genes of anti gastric cancer McAb 3H11 were cloned by RT PCR method from 3H11 hybridoma cells,using 5′ primers for leader sequences.DNA sequences analysis indicated that the cloned genes included the whole leader sequences and the mature Ig protein encoding regions.The 3H11 V L gene was inserted into human mouse chimeric light chain expression vector and transfected into murine Sp2/0 myeloma cells.Transient expression of chimeric light chain protein was detected,which indicated that the cloned gene was functioned in expression.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第3期227-230,共4页
Chinese Journal of Microbiology and Immunology
基金
国家863项目资助
