摘要
本文报道以^(125)Ⅰ-兔网织红细胞5srRNA温育小鼠骨髓瘤细胞SP2/0后的放射自显影实验,及对宿主细胞DNA合成的影响。结果表明,进入小鼠骨髓瘤细胞中的兔网织红细胞5srRNA集中在核内,~3H-TdR参入实验表明,兔网织红细胞5srRNA进入SP2/O核内后,显著抑制了核内DNA合成和肿瘤细胞的分裂活动。以上结果对哺乳类网织红细胞胞质中存在调控肿瘤细胞生长的负调节因子提供了实验依据。
5srRNA was isolated and purified from the reticulocytes of rabbit. It labeled with ^(125)I, then incubated with mouse myeloma cells (SP2/0). By autoradiography it was observed that ^(125)I-SsrRNA could pass into the nuclei of the cells.
In a separate experiment, it showed that the incorporation rate of ~3H-TdR into nuclei of SP2/0 after incubation with 5srRNA was decreased as compared with that f control group, hence the result indicates that 5srRNA inhibits DNA synthesis of the SP2/0 cells and it seems to play a role in the regulation of gene expression through its hybridization with DNA sequences of the SP2/0 cells.
Thus it is likely that 5srRNA might act as 'erythroid denucleation factor'.
出处
《解剖学报》
CAS
CSCD
北大核心
1992年第2期164-168,共5页
Acta Anatomica Sinica
基金
国家科委协调攻关局基金
关键词
网织红细胞
DNA合成
肿瘤细胞
Reticulocytes 5srRNA
DNA synthesis
Autoradiography
~3H-TdR
