摘要
采用电镜、ＤＮＡ琼脂糖电泳和ＦＡＣＳ分析的方法，对枸杞多糖（ＬＢＰ）对小鼠胸腺细胞凋亡的效应进行了研究。结果发现１００μｇ／ｍｌ和４００μｇ／ｍｌＬＢＰ可明显抑制小鼠胸腺细胞培养７小时和２４小时自发出现的细胞凋亡（ａｐｏｐｔｏｓｉｓ），而对１０－７ｍｏｌ／Ｌ地塞米松诱导的细胞凋亡则没有影响。说明ＬＢＰ具有抑制小鼠胸腺细胞自发凋亡的作用，显示出枸杞多糖的一种新的免疫调节效应。
We investigated the effect of LBP on apoptosis of murine thymocyte with electron microscope,DNA agarose electrophoresis and flow cytometry analysis.The results showed that spontaneous apoptosis was markedly inhibited by 100μg/ml and 400μg/ml LBP in murine thymocytes cultured 7h and 24h,but the apoptosis induced by 10 -7 mol/L DEX was not affected by LBP.The results suggested that LBP have the inhibitory effect on spontaneous apoptosis of murine thymocytes.It showed another new immunoregulatory effect of LBP,which played the role on studying mechanism of LBP.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第3期204-207,共4页
Chinese Journal of Microbiology and Immunology
关键词
枸杞多糖
胸腺细胞
细胞凋亡
Thymocyte Lycium barbarum polysaccharide Apoptosis
