摘要
[目的]研究植酸抗油脂过氧化和体外清除自由基能力。[方法]以BHT为对比,采用烘箱法测定植酸的抗油脂过氧化能力。通过Fenton反应体系产生羟自由基(.OH),由连苯三酚在碱性溶液中自氧化反应产生超氧阴离子自由基(O2-.),用分光光度法检测植酸对.OH和O2-.的作用。[结果]植酸能有效地减缓由烘箱法引发的油脂过氧化反应,效果较BHT好,且随着其浓度的增大,POV值的减少,其抗氧化效果越明显。植酸对.OH和O2-.都具有明显的清除作用,且呈一定的量效关系,对.OH和O2-.清除活性IC50值分别为2.54和9.28 mg/ml,对.OH的清除作用强于O2-.。[结论]植酸是一种较好的天然抗氧化剂和自由基清除剂。
[Objective] The aim was to study the capability for anti-axunge peroxidation of phytic acid and removing free radical in vitro.[Method] With BHT as contrast,the capability of anti-axunge peroxidation of phytic acid was measured by using oven method.The hydroxyl radical（·OH）was produced through Fenton reaction system,and the superoxide anion radical（O2·）was produced from self-oxidation reaction in alkalescent solution with the help of pyrogallol,the effect of phytic acid on ·OH and O2^-·was measured with spectrophotometry.[Result] The phytic acid could effectively slow down the axunge peroxidation reaction caused by oven method and got better effect than that of BHT.The antioxidant effect of phytic acid would be more evident along with the increase of its concentration and decrease of POV value.The phytic acid had obvious elimination effect on both ·OH and O2^-· and showed a certain dose-effect relationship.Its IC50 value of scavenging activity against ·OH and O2^-·were 2.54 and 9.28 mg/ml resp.and the scavenging effect to ·OH was better than that to O2^-· [Conclusion] Phytic acid was a good natural antioxidant and radical scavenger.
出处
《安徽农业科学》
CAS
北大核心
2008年第15期6521-6523,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省十一五攻关重点项目(06013046A)
关键词
植酸
过氧化值
羰基值
羟自由基
超氧阴离子自由基
Phytic acid
Peroxide value
Varbonyl value
Hydroxyl radical
Superoxide anion radical
