摘要
目的：探讨乳腺癌癌基因和抑癌基因表达与预后的关系。方法：应用组织原位杂交及免疫组化技术对２５例新鲜乳腺癌标本的ｃ－ｅｒｂＢ－２、ｎｍ２３基因扩增及产物表达进行了检测。结果：ｃ－ｅｒｂＢ－２、ｍＲＮＡ杂交信号主要位于癌细胞胞浆中，杂交颗粒的多少不均，阳性细胞呈异质性分布，ｃ－ｅｒｂＢ－２蛋白在该组表达率为４０％（１０／２５），ｃ－ｅｒｂＢ－２蛋白阳性颗粒分布于癌细胞膜上，随着肿瘤恶性程度的增加，ｃ－ｅｒｂＢ－２基因扩增及蛋白表达都增加（Ｐ＜０．０５）。ｎｍ２３基因扩增及产物表达却随肿瘤恶性程度的增加而减少（Ｐ＜０．０５），ｎｍ２３ｍＲＮＡ杂交颗粒位于胞浆中，蛋白的表达可见于胞浆中，少数见核中阳性，该组ｎｍ２３蛋白表达率为４４％（１１／２５）。结论：ｃ－ｅｒｂＢ－２、ｎｍ２３基因扩增及产物表达呈负相关关系（Ｐ＜０．０５）。
Purpose To examine the expansion and overexpression of oncogenes in breast carcinoma and their relationship to clinicopathologic features and the prognosis. Methods c erbB 2、nm23 oncogene expansion and corresponding oncoprotein were studied in 25 cases of fresh breast carciomas with tissue hybridization in situ and immunohistochemistry methods. Results c erbB 2 mRNA positive grains were mainly in cytoplasm and in an anologus manner, while the positivity rate of c erbB 2 oncoprotein was 40%(10/25) and the protein grains in the cell membrane. The c erbB 2 mRNA and oncoprotein were expressed higher as the carcinoma became worse differentiation ( P <0 05). nm23 mRNA positive grains were found among the cytoplasm and its oncoprotein also mainly in cytoplasm with 44%(11/25) positivity rate. c erbB 2 and nm23 genes expansion and oncoprotein expression were in negative relation. Conclusions nm23 gene expansion and oncoprotein expression are good signs, while the c erbB 2 mRNA and oncoprotein expression are bad signs for the prognosis of the patients and both markers are associated with the pathological behaviour of the breast carcinomas.
出处
《临床与实验病理学杂志》
CAS
CSCD
1997年第3期221-223,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
卫生部青年科学基金
