摘要
运用响应面法对一株枯草芽孢杆菌(Bacillus subtilis)发酵培养条件进行了优化,这株枯草芽孢杆菌产弹性蛋白酶的最适发酵条件为:1.5%葡萄糖、1.5%酵母膏,起始pH为6.5,最适发酵温度为36.5℃,并对由这株枯草芽孢杆菌发酵生产的弹性蛋白酶进行了研究,发现由该株菌发酵得到的弹性蛋白酶在碱性条件下活性较好;在37℃以下时稳定性较好。
Response surface methodology（RSM） was used to optimize the fermentation condition of one kind of Bacillus subtilis,the best fermentation condition of this Bacillus subtilis to produce elastase was： 1.5% glucose, 1.5% yeast extract,the initiation pH was 6.5,the best fermentation temperature was 36.5℃.The properties of the elastase produced by this Bacillus subtilis was also studied,and it showed that the elastase had better activity on the alkaline condition,the stability was better when below 37℃.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第6期142-144,共3页
Science and Technology of Food Industry
