摘要
目的研究脑损伤后,水通道蛋白4(AQP4)的表达变化与血脑屏障(BBB)通透性之间的关系。方法健康成年Wistar大鼠,随机分成创伤性(TBI)组和假手术(SO)组。自由落体硬膜外撞击方法致重度脑创伤模型。于伤后4 h、8 h、12 h、1 d、3 d、5 d、7 d取出大鼠脑组织,进行以下实验:①测创伤脑组织中伊文思蓝(EB)外渗的量,以EB外渗的量反应BBB通透性的变化;②免疫组化(IHC)和原位杂交(ISH)检测AQP4的表达变化。结果脑损伤后,BBB通透性增加,其增加有两个高峰,分别在TBI后12 h和3 d,后者尤为更明显。IHC和ISH显示,脑损伤后AQP4在脑组织中的表达逐渐上调,1 d达高峰,持续至3 d后下降,7 d接近SO组水平。AQP4的表达变化与脑组织伊文思蓝(EB)含量的变化呈正相关(r=0.894,P<0.05)。结论脑损伤后BBB通透性的增加与脑水肿的形成密切相关。TBI后BBB通透性增加,可能与AQP4表达上调有关,两者的变化影响TBI后脑水肿的发生、发展。
Objective To investigate the relationship between the expression of aquaporin-4 （AQP4） and the permeability of blood brain barrier （BBB） after traumatic brain injury （TBI）. Methods A total of 80 healthy Wistar rats were randomly divided into TBI group and sham operation （SO） group. The TBI group was subjected to traumatic brain injury by the Feeney＇s method. The brains of the animals were taken out in 4 h, 8 h, 12 h, 1 d, 3 d, 5 d and 7 d after TBI. Subsequently, the BBB permeability of traumatic brain was determined by Evan blue （EB） extravasation method. The expressions of AQP4 protein and mRNA were detected with IHC and ISH. Results The permeability of BBB increased after TBI. The increasing permeability reached two peaks in 12 h and 3 d post TBI respectively, and the latter was higher. The expression of AQP4 was positive in the cytoplasm and cytomembrane. After TBI, the expression of AQP4 was up-regulated and finally reached a peak in 1 to 3 d post TBI. The permeability of BBB correlated with AQP4 expression positively. Conclusion The permeability of BBB increases after TBI, which implies that the damage of BBB is critical to the formation of brain edema after TBI. The increasing permeability of BBB may be induced by the up-regulated expression of AQP4. The changes of BBB permeability and AQP4 expression may be involved in the formation of brain edema after TBI.
出处
《中华神经外科疾病研究杂志》
CAS
2008年第3期205-208,共4页
Chinese Journal of Neurosurgical Disease Research
关键词
创伤性脑损伤
血脑屏障
AQP4
Traumatic brain injury
Blood brain barrier
Aquaporin-4
