摘要
目的研究DNATyperTM15试剂盒在法医学中的应用。方法共收集60种8137份检材,在34个实验室,用5种不同方法提取DNA,使用DNATyperTM15试剂盒与2种同类进口试剂扩增并对比检测。结果DNATyperTM15试剂盒对不同提取方法、检材种类、扩增与检测仪器、操作环境、操作人员等均具有适应性。结论DNATyperTM15试剂盒能够用于法医学检验鉴定。
Objective To study the application of DNATyperTM15 kit in forensic medicine. Methods Collecting 60 kinds of different testing material totally 8137 samples in 34 labs, Using 5 kinds of different methods to extract DNA, Using the DNATyperTM15 kit to amplify and inspect, Comparing with the other two imported kits of the same kind. Results DNATyperTM 15 kit is suitable to the different extract methods, testing material, instrument of the amplification and inspection, operation environment and the operator. Conclusion DNATyperTM15 kit can be applied in forensic test and identification.
出处
《中国法医学杂志》
CSCD
2008年第3期169-171,共3页
Chinese Journal of Forensic Medicine
基金
国家"十五"功关资助项目(2001BA801B02)
