摘要
对中国西南地区7个黄牛品种81头公牛在4个Y染色体特异微卫星的多态性进行了研究,发现4个微卫星中只有2个,即UMN2404和UMN0103具有多态性,UMN2404具有普通牛(104、91 bp)和瘤牛(120、110和85bp)所特有的单倍型,UMN0103也呈现出能够区分普通牛(155、140 bp)和瘤牛(1361、25 bp)的单倍型,2个标记对不同品种或个体的鉴别一致率达到100%。通过对UMN2404和UMN0103的分析揭示了西南地区黄牛中普通牛和瘤牛Y染色体的分布频率,瘤牛Y染色体单倍型频率(72.8%)显著高于普通牛(27.2%)。普通牛Y染色体单倍型频率在西藏牛(100%)和迪庆牛(81.8%)中占有优势;而瘤牛单倍型在西南地区其他牛种群中占有优势(76.5%~100%)。
Polymorphisms of four Y chromosome microsatellites were analyzed among 81 bulls from 7 indigenous cattle breeds from southwest region of China. Only two out of the five microsatellites exhibited polymorphisms in the bulls analyzed. UMN 2404 showed specific Bos taurus (taurine) (104, 91 bp) and Bosindicus (indicine) (120, 110, 85 bp) haplotypes in these breeds, similarly, UMN 0103 also displayed specific taurine (155, 140 bp) and indicine (136, 125 bp) haplotypes consistent with UMN2404 in each bull examined. Based on the investigation of UMN2404 and UMN0103, we revealed the distribution and genetic introgression of male indicine and taurine in southwest region of China. The frequency of indicine Y specific haplotype (72.8%) was significantly higher than that of taurine haplotype (27.2%). The taurine haplotype dominated in Tibetan (100%) and Diqing cattle (81.8%), while the indicine dominated in the other breeds (76.5%-100%).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第6期705-708,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
西南科技大学博士研究基金项目(06ZX7121)
关键词
黄牛
Y染色体微卫星
多态性
基因流
cattle
Y chromosome specific microsatellites
polymorphism
genetic introgression