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四川黄牛品种线粒体DNA遗传多样性研究 被引量:27

Study on Mitochondrial DNA Genetic Polymorphism of Cattle Breeds in Sichuan Province
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摘要 测定了四川黄牛5个品种的线粒体细胞色素b部分片段(420 bp)42个个体以及28个个体线粒体DNA控制区(D-loop)的全序列,结合已报道的中国8个黄牛品种22个个体的线粒体DNA控制区(D-loop)全序列,分析结果表明:四川黄牛的部分Cyt b基因片段有5个变异位点,7种单倍型可分为以2个单倍型为主的2大类型;线粒体DNA控制区检测到64个变异位点,28种单倍型,单倍型H1~H23为普通黄牛类型,单倍型H24~H28为瘤牛类型的单倍型.在四川黄牛中,67.86%为普通牛类型,32.14%为瘤牛类型.研究结果表明四川黄牛主要有2类母系来源. This study sequenced partial sequences of mitochondrial DNA Cyt b gene (420 bp) of 42 individuals and D-loop region (910 bp) of 28 individuals in 5 cattle breeds from Sichuan Province. The results showed that there were 5 mutation sites among the partial Cyt b gene and 7 haplotypes, which was divided into two main types. There were 64 mutation sites and 28 haplotypes defined in D-loop region when the 22 published Chinese cattle D-loop sequences aligned together with the data in this study. Haplotype H1 -H23 was belonging to Bos. Taurus type while Bos. Indicus type contained haplotypes from H24 to H28. 67.86% individuals were Bos. Taurus type and 32.14% were Bos. Indicus type in Sichuan cattle breeds. It suggested that the Sichuan cattle were mainly origined from Bos. Taurus type and Bos. Indicus type.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第9期887-892,共6页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 国家"十五"科技攻关项目(2002BA514A23) 四川"十五"科技攻关项目
关键词 黄牛 线粒体DNA D-LOOP 起源 cattle mitochondrial DNA D-Loop origin
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参考文献13

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