摘要
以黑曲霉T-0218为发酵菌种，进行了以木薯为原料生产柠檬酸的发酵条件研究及工业化放大试验．确定了最佳的发酵工艺：发酵培养基中碳源质量浓度≤200g／L，接种菌丝球数量≥10000mL^-1，种龄16～24h．在最佳的发酵工艺条件下，放大试验取得了满意的结果，其生产指标：产酸141g／L，周期60．3h，转化率100．4％，发酵指数2．34g·（L^-1·h^-1）．
The citric acid production by strain Aspergillus niger T-0218 by using cassava and industrial scale-up were investigated. The best conditions was obtained under the following fermentation conditions：concentration of fermentation medium was less than 200 g/L, the number of mycelium pellets were more than 10 000/ml and age of seed wasl6 hours to 24 hours.Under the best conditions, good results were obtained in the industrial scale-up experiments, they are following： CAM 141 g/L; period 60.3 h; yield 100.4%; productivity 2.34 g · （L^-1 · h^-1）.
出处
《天津科技大学学报》
CAS
2008年第2期43-46,共4页
Journal of Tianjin University of Science & Technology
关键词
黑曲霉
木薯
柠檬酸
发酵
Aspergillusniger
cassava
citric acid
fermentation
