摘要
目的对胶乳增强免疫比浊法定量测定血清Ⅳ型胶原蛋白的方法进行临床实验评价。方法实验评价包括精密度、线性测定、前带反应、相关性、回收实验以及干扰实验。结果胶乳增强免疫比浊法测定血清Ⅳ型胶原蛋白批内精密度测定CV%为2.47%～3.48%,批间精密度测定CV%为3.46%～4.07%,回收率为96.2%～99.3%。线性测定良好,可接受范围为800ng/mL,回归方程为Y=1.0118X-1.7883,不同质量浓度的标准品检测相关性r2=0.9999,Y=1.0031X-1.2362。对胆红素、血红蛋白、甘油三酯均具有很好的抗干扰能力。结论利用胶乳增强免疫比浊法在全自动生化仪上测定血清Ⅳ型胶原蛋白的结果符合临床使用的要求。
Objective To appraise determination of serum type Ⅳ collagen protein with latexenhanced immunoturbidimetry. Methods The evaluation test was performed including precision, linearity determination, prozone reaction, correlation, recovery test and interference test. Results The intra-bateh CV% was within 2.47%-3.48%, inter-batch CV% was within 3.46%-4.07%, and recovery rate was within 96.2%-99.3%. There was a good linearity, and the tolerance limit was 800 ng/mL. Regression equation：Y= 1. 011 8X-1. 788 3 ;detection correlation of different concentrations of stand- ard preparations：re =0. 999 9,Y= 1. 003 1X-1. 236 2. The assay had a very good anti-interference performance to billrubin, eruorin, and triglyeeride without exception. Conclusion Determination of serum type Ⅳ collagen protein attains the demand of the clinic with latex-enhanced immunoturbidimetry in fully automatic biochemical analyzer.
出处
《国际检验医学杂志》
CAS
2008年第6期508-509,共2页
International Journal of Laboratory Medicine
关键词
散射测浊法和比浊法
胶原Ⅳ型
肝硬化
评价研究
Nephelometry and Turbidimetry
Collagen, Type Ⅳ
Liver Cirrhosis
Evaluation Studies
