摘要
目的:研究反应停(thalidomide)对碱烧伤诱导角膜新生血管(corneal neovascularization,CNV)的影响。方法:建立大鼠角膜碱烧伤诱导CNV模型,SD大鼠36只随机分成角膜新生血管对照组(A组)、反应停灌胃组(B组)和反应停球结膜下注射组(C组)。采用裂隙灯照相、免疫组化和RT-PCR等方法,检测碱烧伤后各时间点不同处理方法大鼠CNV的生长情况及VEGF的表达情况。结果:B组和C组的角膜新生血管面积和VEGF的表达均显著少于A组(P均(0.05);C组的角膜新生血管面积和VEGF的表达少于B组(P均<0.05)。结论:反应停可有效抑制大鼠角膜碱烧伤诱发CNV的生长,降低角膜新生血管VEGF的表达。球结膜下注射较全身应用更有效。
AIM： To investigate the effects of thalidomide on corneal neovascularization induced by alkali burn.
METHODS： CNV model was made by alkaline burning. Thirty-six experimental rats were randomly divided into CNV control group （group A）, thalidomide group treated orally with 200mg/（ kg·d） （ group B） and subconjunctivalinjected group of thalidomide（ group C）. The development of CNV was observed by slit lamp biomicroscopy, photography in different groups. Immunohistochemisty and reverse transcription-polymerase chain reaction （ RT- PCR） were used to determine VEGF mRNA level and protein level, respectively.
RESULTS： Compared with the control group（group A）, the corneal neovascularization area and the expression of VEGF decreased in group B and C （P〈0.05）. The corneal neovascularization area and the expression of VEGF in group C was lower than that in group B （P〈0.05）.
CONCLUSION： Thalidomide can inhibit the growth of CNV induced by alkaline buring in rats effectively, and reduce the expression of VEGF in CNV. Subconjunctival injection of thalidomide is a more effective angiostatic approach.
出处
《国际眼科杂志》
CAS
2008年第6期1146-1149,共4页
International Eye Science
关键词
反应停
角膜新生血管
血管内皮生长因子
thalidomide
corneal neovascularization
vascular endothelial growth factor
