摘要
目的: 探讨99Tc-MDP对碱烧伤性大鼠角膜新生血管生成的抑制作用。方法: 采用碱烧伤法制备角膜新生血管模型,24只健康SD大鼠随机分成两组,实验组予以腹腔注射99Tc-MDP,每天1次,至模型建立后14d。对照组以等体积的生理盐水腹腔注射。观察新生血管形成时间、第21d时角膜新生血管长度和新生血管面积。结果: 实验组新生血管长入的时间为6.25±1.93d,对照组长入时间为5.83±1.86d,差异无统计学意义(P=0.30)。新生血管长度实验组为1.74±0.33mm,对照组为2.82±0.25mm;新生血管的面积实验组为17.3±3.26mm2,对照组为24.8±5.10mm2,两者之间差异均有统计学意义(P<0.01)。结论: 使用99Tc-MDP可以有效抑制碱烧伤引起的角膜新生血管的生成。
·AIM： To evaluate the effect of inhibition of 99Tc-MDP on experimental corneal neovascularization in rats.·METHODS： Chemical cauterization of the cornea was performed by touching alkali. Twenty-four healthy SD rats were divided randomly into two equal groups： experimental group underwent i. p. injection of 99Tc- MDP everyday until 14th day after alkali burn. Control group underwent i. p. injection of normal saline as control. Rats were examined for detection of the first signs of neovascularization. 21 days later, lengths of neovascular and total corneal neovascularization area were assessed.· RESULTS： The time of neovascularization in experimental group was 6.25±1.93 days, the control group was 5.83±1. 86 days, and there was no statistically significant difference（ P= 0.30）. The lengths of neovascular were 1.74±0.33mm and 2.82±0.25mm in two groups and the difference was statistically significant. The total neovascularization area was 17.3±3.26mm2, 24.8±5.10mm2 respectively and the difference between the two groups was statistically significant （ P〈0.01 ）.·CONCLUSION： 99Tc-MDP has a significant effect on inhibition of alkali burn-induced corneal neovascularization.
出处
《国际眼科杂志》
CAS
2013年第8期1547-1549,共3页
International Eye Science
