摘要
采用线栓法制成大鼠大脑中动脉梗塞 ( MCAO)模型 ,依 Hb O2 - NO法测定持续性脑缺血和缺血 /再灌流脑组织内 NO含量的变化 ,以探讨不同脑缺血和再灌流过程中 NO的变化规律及其意义。结果 :缺血 3小时受损脑组织 NO水平即增高 ,再灌流后 NO逐步升高 ,而持续性缺血状态下 NO则表现降低后再升高的变化。虽然两组 NO在 7天时均有明显降低 ,但仍高于缺血前水平。认为持续性脑缺血和缺血 /再灌流情况下 NO的变化规律有所不同 ,与缺血脑组织的缺氧及产生 NO所需底物供应缺乏有关 。
The aim of our study wasto investigated the changesof NO concentration in the ischemic and reperfutional brain tissues and the relationship between the NO and brain damage.The animal models of middle cerebral artery occlusion were established by suture method.The changesof NO concentration were researched with method of Hb O2 -NO during the permanent ischemia and ischemia/ reperfusion.The results showed that the NO level in the injured brain tissue after3hours of ischemia was increased.After reperfusion,the NO level increased progressively,but NO level showed decreased and then increased during the permanent ischemia.Although there wasa decrease of NO level at7days after ischemia and reperfusion,but the NO level was higher than that of preischemia.It indicated that the changes of NO concentration during the different process of cerebral ischemia and reperfu- sion are related to the differentisoform of NOS,which have respective characteristic forsyn- thesizing NO in biochemistry,and deal with the cerebral ischemic and reperfusional injury.
出处
《临床神经病学杂志》
CAS
1997年第6期326-329,共4页
Journal of Clinical Neurology
关键词
脑缺血
再灌流
一氧化氮
大鼠
Cerebral ischemia Reperfusion Nitric oxide Rat
