摘要
目的研究高碳酸血症(HPC)对急性肺损伤(ALI)氧化-抗氧化平衡的影响。方法24只SD大鼠随机分为正常对照组(Ⅰ组);ALI组(Ⅱ组);ALI+HPC组(Ⅲ组);每组8只。用0.2M盐酸(2ml/kg)气管内滴入建立大鼠急性肺损伤模型,吸入8%CO2气体建立高碳酸血症模型。以肺组织病理变化、支气管肺泡灌洗液(BALF)细胞计数、肺湿重/干重比(W/D)、肺渗透系数、白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)浓度为评估肺损伤的指标;以肺组织匀浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和血过氧亚硝酸盐(NOx)浓度评估氧化应激水平。结果Ⅲ组BALF细胞计数(1.02±0.48)×109/L、W/D7.24±0.58、肺渗透系数0.25±0.16、IL-8浓度(29.95±7.11)pg/ml和TNFα浓度(83.80±46.93)pg/ml比Ⅱ组相应指标(1.79±0.73)×109/L;8.60±1.24;0.53±0.35;(59.50±36.00)pg/ml、(161.57±54.12)pg/ml明显降低(P<0.01)。血NOx浓度(15.91±4.33)μmol/L和肺组织MDA含量(1.51±0.15)mol/gprot比Ⅱ组相应指标(22.94±4.19)μmol/L、(1.96±0.33)mol/gprot明显降低(P<0.01)。结论HPC对盐酸诱导的大鼠ALI有保护作用,HPC保护内源性抗氧化物质的活性,减少活性自由基的产生,维护氧化抗氧化平衡是其保护作用的重要机制。
Objective To study the effects of hypercapnia on the balance of oxidation- antioxidation in rats with acute lung injury（ALI）. Methods Twenty SD rats were randomized into there groups of normal control （groupⅠ ）, ALI （group Ⅱ ） and ALI＋ hypercapnia （group Ⅲ ）. ALI model was induced by HC1 （0. 2 M） instillated into bronchial （2 ml/kg）. The lung histological changes, wet-to-dry weight ratio, lung permeability coefficient, bronchial alveolar lavage fluid（BALF） cells count, BALF concentration of interleukin 8 （IL-8） and tumor necrosis factor α （TNFα） were used to assess injury, the activity of SOD and the content of MDA in lung tissue and the blood concentration of peroxynitrate（NOx） were used to assess the level of oxidative stress. Results The indices of lung inflammation including BALF cell count [（1.79±0. 73）×10^9/L], the concentration of IL-8 [（59.52±36. 00） pg/ml] and TNF-α [（161.57±54. 12） pg/ml] in BALF, lung permeability coefficient （0.53±0. 35） ,W/D ratio （8.60± 1.24） were significantly higher in group Ⅱ than those [（1.02±0. 48） × 10^9/L, （29.95±7.11） pg/ml, （83. 80±46. 93） pg/ml, （0. 25±0.16） ,and （7.24±0. 58）1 in group Ⅲ （P〈0.01）. The serum concentration of NOx[（22. 94±4.19） μmol/L] and the content of MDA[ （1.96±0. 33） mol/gprot] in lung tissues homogenate were markedly higher in group Ⅱ than those[（15.91±4. 33） μmol/L,（1.51±0. 15） mol/gprot] in group In （P〈0.01）. Conelution On the basis of our experiment, we conclude that hypercapnia has protective effects on ALI induced by HCL through proetecting the activity of endogeneous antioxidative substance and attenuating the product of reactive species.
出处
《江苏医药》
CAS
CSCD
北大核心
2008年第6期608-610,F0003,共4页
Jiangsu Medical Journal
