摘要
[目的]建立糖尿病大鼠难治性溃疡动物模型。[方法]在糖尿病大鼠背部造成圆形无菌创面(直径2cm),分别于1、3、7、14、21 d监测血糖同时进行创面照相,计算机图象分析系统分析溃疡的大小及深度,创面愈合时间。[结果]糖尿病组动物的愈合速度明显低于空白对照组动物,通过对两组肉芽组织的电镜观察糖尿病组动物的成纤维细胞出现的时间和数量明显晚于空白对照组。[结论]以1%键脲菌素能成功建立糖尿病大鼠难治性溃疡动物模型。
[Objection] To dynamic observe the terminal glycosylation production and acceptor of granulation tissue of ulcus raw surface in rats with diabetes and explore the effect mechanism of [Methods] To establish the animal model of diabetes ulcer. The model rats were demixing randomly divided into diabetes control group, control group, Vasogen group, Shengji corium elephatis and obtain fresh granulation tissue by using operation method. To determine the terminal glycosylation production content by using RT-PCR and immunohistochemical method. [Results] The content of terminal glycosylation production wasn＇t obviously differentiation on the rats of every of mean tendency, the content of RAGE in Shengji corium elephatis mastic group was obviously may inhibit the production of RAGE with the prolongation of time. The expression of RAGE was low in rats of every diabetes group in twenty day. [Conclusion] Shengji corium elephatis mastic prolongation of time.
出处
《天津中医药大学学报》
CAS
2008年第2期72-74,共3页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30400605)
关键词
糖尿病
难治性溃疡
动物模型
Shengji corium elephatis mastic
diabetes ulcus
terminal glycosylation production
RAGE
