摘要
目的：测定三个不同产地天麻中天麻素含量。方法：采用HPLC法测定三产地天麻中天麻素的含量。Agilent TC—C18色谱柱（4．6mm×250mm，5μm）；流动相：乙腈-0．05％磷酸（4：96）；流速：1．0mL／min；检测波长：220nm。结果：天麻素在20～200μg的范围内呈现良好的线性关系，平均回收率为97．93％，RSD为1．49％（n=9）。云南昭通、湖北宜昌、陕西西乡三产地天麻中天麻素质量百分含量分别为0．164％，0．263％，0．418％．结论：三产地的天麻中天麻素含量有明显差异，云南昭通产天麻中天麻素含量最低。
Objective： To determine the content of gastrodin in three different habitats/Gastrodia elata Blume. Method： To determine the content of gastrodin in three different habitats/ Gastrodia elata Blume by HPLC, on Agilent TC- C18 column, with Methylcyanide- 0. 05% phosphoric acid （4：96） as mobile phase, 1.0mL/ min as speed of flow, detection wavelength at 220nm. Result ： The good linear correlation was obtained in the range 20 - 200μg. The average recovery of spots （ n = 9） was 97.93 % （ RSD = 1.49 % ）. The content of gastrodin in the Yunnan Zhaotong, the Hubei Yichang, the Shanxi Xi Town respectively was 0. 164%, 0. 263%, 0. 418%. Conclusion： There were obvious differences in the content of gastrodin in three different habitats＇ Gastrodia elata Blume. The content of gastrodin in Yunan Zhaotong＇s Gastrodia elata Blume was the lowest.
出处
《云南中医学院学报》
2008年第3期6-8,共3页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金项目。(NO:30760305。)
关键词
HPLC法
天麻素
含量测定
HPLC
Gastrodia elata Blume
Content Determination
