摘要
目的研究阿魏酸钠对谷氨酸单钠在成年小鼠脑中分布的影响。方法采用同位素示踪法,将小鼠分为对照组:MSG(2.0g/kg体重)灌胃后立即腹腔注射SF(0.08g/kg体重);[3H]Glu组:一次性灌胃给予小鼠含[3H]Glu(640kBq/鼠)的MSG(2.0g/kg体重);[3H]Glu+SF组:一次性灌胃给予含[3H]Glu(640kBq/鼠)的MSG(2.0g/kg体重)后,立即一次性分别腹腔注射SF(0.02、0.04、0.08g/kg体重)。给药后30、60、90、120min每组牺牲4只小鼠,取出全脑,匀浆,测定放射性强度。结果结果表明,在30-120min时段,脑中的[3H]Glu含量呈逐渐上升趋势;同一时间点各组[3H]Glu含量进行比较,[3H]Glu+SF组与[3H]Glu组相比较有明显下降;SF对MSG分布的影响与剂量相关。结论阿魏酸钠显著减少谷氨酸单钠在小鼠脑组织的分布。
Objective To study the effect of sodium fentlate （SF） on monosodium glutamate （MSG） distributing in the adult mouse brain. Methods [^3H]-labeled glutamate （[^3H]Glu） was used as radiotracer to study the effect of SF on distribution of MSG in adult murine brain. The mice were randomly divided into control, [^3H]Glu, and [^3H]Glu＋SF （0.02,0.04,0.08g/kg） groups, n=16. The animals in control group received intragastric （ig） administration of MSG （2.0g/kg） and intraperitoneal （ip） administration of SF （0.08g/kg） simulta- neously; The animals in the [^3H]Glu group received ig administration of MSG （2.0g/kg） containing [^3H]Glu （640kBq/mouse）, the animals in the [^3H]Glu＋SF groups received ig administration of MSG （2.0g/kg） containing [^3H]Glu （640kBq/mouse） and ig administration of SF （0.02, 0.04, 0.08g/kg） simultaneously. The mice were sacrificed by perfusion of physiological saline at 30, 60, 90, and 120 min after the administration of MSG or/ and SE The brains were excised and homogenized carefully, and then the radioactivity in an aliquot of lysate was counted. Results Marked elevations of [^3H]Glu were detected in the murine brains treated with [^3H]Glu or [^3H]Glu＋SF, and the uptake of [^3H]Glu in the brains rose gradually during 30-120 min, but the elevations of [^3H]Glu in the brains treated with [^3H]Glu＋SF were significantly lower than those of [^3H]Glu in the brains treated with [^3H]Glu alone. The results indicated that SF apparently inhibited the uptake of labeled glutamate in brain in a dose-dependent manner. Conclusion The SF obviously inhibited the uptake of glutamate in murine brain.
出处
《海南医学》
CAS
2008年第7期70-71,共2页
Hainan Medical Journal
关键词
阿魏酸钠
谷氨酸的分布
脑组织
小鼠
Sodium ferulate
Distribution of MSG
Brain tissue
Mouse
