摘要
目的:通过建立大鼠高脂血症模型以动态观察其在不同时间点上所致大鼠血管内皮细胞损伤后血浆抗血管性假性血友病因子(von Willebrand’s factor,vWF)、纤溶酶原活化抑制物-1(plasmino-gen activator inhibitor-1,PAI-1)和组织型纤溶酶原激活物(tissue-type plasminogen activator,t-PA)分子标志物的表达。方法:取体质量为(200±20)g健康雄性SD大鼠60只,随机分为6组(10只/组),即高脂合剂灌胃7周组(A7组)、高脂合剂灌胃8周组(A8组)、高脂合剂灌胃9周组(A9组);普通饲料喂养7周组(C7组)、普通饲料喂养8周组(C8组)、普通饲料喂养9周组(C9组)。实验结束时处死各组动物,取血标本,分离血清立即测定血脂;取大鼠主动脉制成病理切片,光镜下观察内皮的病理改变,并另取血浆以酶联免疫吸附法(ELISA)检测血浆vWF,PAI-1和t-PA的含量。结果:⑴7,8,9周高脂合剂灌胃组大鼠血脂TG,TC和LDL均高于其相应对照组,而HDL则先随灌胃时间的增加而升高,后随着灌胃时间的延长再次降低。⑵光镜下检查发现对照组大鼠主动脉内皮细胞形态正常,而各模型组大鼠主动脉内皮细胞有不同程度的损伤。⑶在3个模型组中,血浆中vWF和PAI-1含量随着高脂血症持续时间的延长而增加;t-PA的含量随着高脂血症持续时间的延长而降低;⑷在A7和A8组vWF的表达比较无明显差别,它不随内皮病变的改变而变化;在模型组中,PAI-1随内皮细胞损伤的加重而逐渐增高;在模型组中,t-PA随内皮细胞损伤的加重而逐渐递减。结论:⑴大鼠主动脉内皮细胞随高脂血症持续时间的延长,病理损伤逐渐加重。⑵血浆中vWF,PAI-1和t-PA的改变可作为判断高脂血症大鼠血管内皮细胞损伤的分子标志物。⑶在高脂血症大鼠血管内皮细胞损伤中,反映血管内皮细胞早期的病变PAI-1和t-PA比vWF更敏感。
Objective To establish the hyperlipoidemia model and observe its effect on the expression of plasma vWF, PAl-1 and t-PA after endothelial cell injury caused by the model at different time in rats. Methods Sixty male Sprague-Dawley rats weighing （200 ± 20 ）g were randomly divided into 6 groups （n = 10 in each） ： high flat composition for 7 weeks （Group A7）,8 weeks （ Group A8 ） and 9 weeks （ Group A9 ）, common bait vessel feeding for 7 weeks （ Group C7 ）, 8 weeks （ Group C8 ） and 9 weeks （ Group C9 ）. When it reached the setting time, the rats were put to death and the serum was separated to detect the blood-fat immediately. Meanwhile, the pathological sections of the rat aorta were made to observe the endothelial injury. The blood plasma was separated and the content of the blood plasma PAl-1 , t-PA and vWF was detected by ELISA assay. Results （ 1 ） TG, TC and LDL of the rats intragastrically administrated high fiat composition were higher than those of the control group, and HDL increased with time at first, but lowered when longer than 9 weeks. （2） There were no lesions in the aortic endothelium in all rats of Group C through optical microscope, while different lesions in the aortic endothelium in all rats of Group A were observed. （3） In the 3 groups A, the plasma vWF and PAI-1 were gradually increasing with time while t-PA gradually decreased. （4） There was no significant difference of vWF between Group A7 and Group A8. PAI-I increased gradually with the aggravation of endotheliocytes in Group A; and t-PA de- creased gradually with the aggravation of endothelium in Group A. Conclusion （ 1 ） As intragastric administration prolongs, the plasma lipid of rats keeps increasing with the aggravation of the endoder- mis injury. （2） PAI-1 , t-PA and vWF are the molecular markers of vascular endothelial cell injury in rats. （3） PAI-I and t-PA are more sensitive than vWF in reflecting the endothelial cell injury in hyperlipemia early metaphase.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2008年第5期415-420,共6页
Journal of Central South University ：Medical Science
基金
湖南省自然科学基金(02jjy2053)~~
