摘要
目的探讨小陷胸汤对高血脂症(hyperlipemia,HLP)小鼠血管内皮损伤的影响。方法选用36只C57BL/6小鼠,随机分为对照组,模型组,低剂量组(0.03 g/mL小陷胸汤)、中剂量组(0.06 g/mL小陷胸汤)、高剂量组(0.12 g/mL小陷胸汤)和洛伐他丁组(2.5 mg/kg洛伐他丁),每组6只,通过高脂饲料喂养8周建立HLP模型并给予相应药物进行干预。给药期间观察各组小鼠一般行为学状态,每周称重一次;HE染色观察主动脉血管组织病理学变化;免疫组化法检测小鼠主动脉组织血管假性血友病因子(von Willebrand factor,vWF)的表达;q RT-PCR检测小鼠主动脉组织血管内皮生长因子(vascular endothelial growth factor,VEGF)、酪氨酸蛋白激酶Eph受体B4(EPH receptor B4,EphB4)及其胞膜附着型配体B2(Ephrin B2)的m RNA水平;Western blot检测小鼠主动脉组织VEGF、Eph B4和Ephrin B2蛋白水平。结果与对照组相比,模型组小鼠活动量和摄食量减少,毛发较差,体重增加,主动脉组织中v WF、VEGF、Eph B4、Ephrin B2表达水平升高;与模型组相比,小陷胸汤各剂量组和洛伐他丁组小鼠活动量和摄食量增加,毛发光亮,体重减少,主动脉组织中v WF、VEGF、Eph B4、Ephrin B2表达水平降低(P<0.05);与低剂量组比较,中、高剂量组小鼠主动脉组织中VEGF、Eph B4和Ephrin B2 m RNA及蛋白表达水平降低(P<0.05)。结论小陷胸汤可抑制HLP的发展,对小鼠血管内皮具有保护作用。
Objective To explore the effect of Xiaoxianxiong Decoction on vascular endothelial injury in mice with hyperlipidemia(Hyperlipemia,HLP).Methods Thirty-six C57 BL/6 mice were randomly divided into control,model,low dose group(0.03 g/mL Xiaoxianxiong Decoction),medium dose(0.06 g/mL Xiaoxianxiong Decoction),high dose group(0.12 g/m L Xiaoxianxiong Decoction),and Lovastatin(2.5 mg/kg lovastatin)groups.Six rats in each group were fed a high fat diet for 8 weeks to establish the HLP model and administered corresponding drugs for treatment.During the administration period,the general behavioral state of mice in each group was observed and mice were weighed once a week.HE staining was used to observe histopathological changes of aortic vessels.Immunohistochemistry was used to detect expression of von Willebrand factor(vWF)in aortic tissues of mice.q RT-PCR was used to measure m RNA levels of vascular endothelial growth factor(VEGF),EPH receptor B4(Eph B4),and Ephrin-B2(Ephrin B2)in aortic tissues of mice.Western blot was used to measure the protein levels of VEGF,Eph B4,and Ephrin B2 in aortic tissues of mice.Results Compared with the control group,the activity and food intake of mice in the model group was decreased,hair was poor,weight had increased,and the expression levels of v WF,VEGF,Eph B4,and Ephrin B2 were increased in aortic tissue.Compared with the model group,the activity and food intake of mice in each dose group of Xiaoxianxiong Decoction and the Lovastatin group were increased,hair was brighter,weight was reduced,and the expression levels of v WF,VEGF,Eph B4,and Ephrin B2 were decreased in aortic tissue(P<0.05).Compared with the low dose group,m RNA and protein expression levels of VEGF,Eph B4,and Ephrin B2 were decreased in medium and high dose groups(P<0.05).Conclusions Xiaoxuai decoction inhibits the development of HLP and protects the vascular endothelium of mice.
作者
曾江琴
孙勤国
徐鸿婕
丁晓明
牟艳杰
蒋跃文
ZENG Jiangqin;SUN Qinguo;XU Hongjie;DING Xiaoming;MOU Yanjie;JIANG Yuewen(Department of Traditional Chinese Medicine,Wuhan Third Hospital,Wuhan 430060;China.2.Hubei University of Chinese Medicine,Wuhan 430061)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第10期48-53,共6页
Chinese Journal of Comparative Medicine
基金
武汉市卫生计生委科研计划资助项目(WZ18A01)。
关键词
小陷胸汤
高血脂症
血管内皮
Xiaoxianxiong Decoction
Hyperlipidemia
Vascular endothelium
