摘要
[目的]为南瓜属植物优良品种的快速繁育、抗逆性品种的选育等提供研究基础。[方法]以"锦粟2号"南瓜叶片为外植体,用不同植物生长调节物质进行组织培养和植株再生。[结果]结果表明:试验条件下,诱导叶片分化形成愈伤组织的最佳培养基为MS+6-BA1.0 mg/L+NAA 0.1 mg/L;最适的芽分化培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L;诱导芽生根的最佳培养基为1/2 MS+NAA 0.5mg/L,芽生根率为100%。[结论]成功获得了"锦粟2号"南瓜的愈伤组织及再生植株。
[ Objective] The research aimed to provide foundation for the rapid propagation of superior variety and the selection of resistant variety of Cucurbita. [Method] The leaves of ＂Jinsu 2＂ was used as the explant and treated with different plant growth regulators in tissue culture and plantlet regeneration. [Resuhs] The best culture for leaves callus inducement was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.1 mg/L. The most suitable culture for bud rooting inducement was 1/2 MS ＋ NAA 0.5 rag/L, and the percentage of rooting was 100%. [ Conclusion ] Tissue culture and plantlet regeneration of ＂Jinsu 2＂ was obtained successfully.
出处
《安徽农业科学》
CAS
北大核心
2008年第13期5311-5312,5448,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省科技厅计划项目(2005C24009)
关键词
南瓜
组织培养
植株再生
Pumpkin
Tissue culture
Plantlet regeneration
