摘要
目的:建立空心莲子草药材的定性定量方法。方法:采用 TLC 法进行定性鉴别,以三氯甲烷-甲醇(40:1)为展开剂;采用 HPLC 法测定齐墩果酸的含量,色谱柱为 Alltima C_(18)(250 mm×4.6 mm,5μm),以甲醇-水-冰醋酸-三乙胺(90:10:0.03:0.06)为流动相,流速0.6 mL·min^(-1),检测波长210 nm,柱温25℃。结果:空心莲子草薄层色谱鉴别专属性强;齐墩果酸进样量在1.03～4.99μg范围内与峰面积呈良好的线性关系,线性回归方程为 Y=3.676×10~3X-3.958×10~3(r=0.9997),平均回收率(n=6)为99.8%(RSD=2.3%)。结论:本方法简便、准确,重复性好,能有效控制空心莲子草药材质量。
Objective：To establish qualitative and quantitative methods of Alternanthera philoxerodes. Method： Qualitative analysis for Alternanthera philoxerodes by TLC ;the chromatographic condition for Alternanthera philoxerodes were：a developer of chloroform - methanol （40：1 ）. The content of oleanolic acid in Alternanthera philoxerodes was determined by HPLC ,Alltima C18 colunm（250 mm ×4.6 mm,5 μm）was used. The mobile phase was a mixture of methanol - water - glacial acetic acid - triethylamine （90： 10： 0. 03： 0. 06 ） at a flow rate of 0.6 mL · min^ -1, column temperature was 25 ℃, and the detection wavelength was 210 nm. Results ：Alternanthera philoxerodes could be detected by TLC ; the calibration curve of oleanolic acid was linear over the range of 1.03 - 4.99 μg, and the equation of linear regression was Y = 3. 676 × 10^3X - 3. 958 × 10^3 （ r = 0. 9997 ）. The average recovery rate （ n = 6） was 99.8%, RSD = 2. 3%. Conclusion：The method is convenient, accurate and have a good reproducibility. The quality of Alternanthera philoxerodes can be controlled effectively.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期732-734,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金资助项目(30470194)
关键词
空心莲子草
齐墩果酸
定性鉴别
HPLC
含量测定
Alternanthera philoxerodes
oleanolic acid
identification
HPLC
content determination
