摘要
目的:研究甲状腺组织中CD151mRNA和蛋白的表达及其与甲状腺肿瘤的关系。方法:用逆转录-聚合酶链反应(RT-PCR)及免疫组化EnvisionTM二步法检测27例甲状腺癌、10例滤泡性腺瘤及8例正常甲状腺组织中CD151 mRNA及蛋白的表达,并与临床及病理资料进行比较。结果:CD151mRNA和蛋白在甲状腺癌组的表达明显高于滤泡性腺瘤组和正常甲状腺组,滤泡性腺瘤组高于正常甲状腺组,组间比较均有统计学差异(P<0.01;P<0.05);CD151mRNA和蛋白表达与甲状腺癌患者的年龄、性别、肿瘤大小及淋巴结转移无相关性(P>0.05);CD151mRNA和蛋白在甲状腺病变组织中的表达呈正相关(r=0.511;P<0.01)。结论:CD151在正常甲状腺、滤泡性腺瘤及甲状腺癌中的表达呈递增趋势,提示CD151的高表达可能在甲状腺肿瘤的发生发展中起促进作用。
Objective：To study the gene and protein expression of CD151 in thyroid tissues and its relation to thyroid tumor. Methods： The gene and protein expression of CD151 in thyroid tissues were detected by RT - PCR and immunohistochemistry, including 27 cases of carcinoma, 10 cases of thyroid adenoma and 8 normal tissues of thyroid, and compare the clinical and pathological data. Results： The expression of CD151 in thyroid carcinoma was obviously higher than other two groups and thyroid carcinoma was higher than normal tissues （ P 〈 0.01, P 〈 0.05 ）, but it has not correlation with age, gender, cancer diameter and lymph node metastasis （ P 〉 0.05 ）. Further more the expression of gene in accordance with protein（ r =0. 511 ,P 〈0.01 ）. Conclusion：The expression of CD151 in normal tissues of thyroid, thyroid adenoma and thyroid carcinoma increases progressively, suggests that CD151 promotes the development of thvroid tumors.
出处
《现代肿瘤医学》
CAS
2008年第6期929-932,共4页
Journal of Modern Oncology
基金
广西自然科学基金项目资助(桂科自0640112)
