摘要
采用滤纸片法,检测不同质量浓度马尾松Pinus massoniana叶提取物对金黄色葡萄球菌Staphylococcus aureus,大肠杆菌Escherichia coli,枯草芽孢杆菌Bacillus subtilis,八叠球菌Sarcina ventriculi,木霉Trichoderma viride,青霉Penicillium chrysogenum和黑曲霉Aspergillus niger等的抑制效果。结果表明,马尾松叶的水、丙酮、乙醇溶液提取物对细菌的抑菌圈直径随着提取物质量浓度的增大,对各种菌的抑菌效果越好,马尾松叶乙醇提取物质量浓度为100.0 g.L-1时,大肠杆菌抑菌圈直径为25.6 mm;对3种霉菌的抑菌效果与质量浓度的大小不成正比;3种提取物中以乙醇提取物的抑菌效果最好,而水提物的抑菌效果较差。
The antimicrobial activity of ethanol, acetone, and aqueous extracts from Pinus massoniana needles on four bacteria （Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Sarcina ventriculi ） and three mold fungi （Trichoderma viride, Penicillium chrysogenum, and Aspergillus niger） was evaluated by agardiffusion bioassasy. Filter paper discs impregnated with extracts at concentrations of 100. 0, 50.0, 25.0, 12.5, 6. 3, 3.1, 1.6 g· L-1 were placed on the agar surface. Reversal of the antiviral activity of mycophenolic acid was indicated by virus breakthrough in those cells in close proximity to the filter paper disc. All extracts, depending on their concentrations, showed different resistances to bacteria; however, their resistance to fungi differed. The assay showed that a 100.0 g· L-1 ethanol extract concentration of P. massoniana needles had a 25.6 mm diameter zone of inhibition against E. coli. For the three kinds of extracts, ethanol had the highest resistance and aqueous the lowest. The results shows that the extracts of P. massoniana needles have antimicrobial activity. [ Ch, 2 fig. 1 tab. 9 ref.
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2008年第3期359-362,共4页
Journal of Zhejiang Forestry College
基金
浙江省科学技术攻关项目(2005C12022)
关键词
森林保护学
马尾松叶
提取物
抗菌活性
forest protection
Pinus massoniana needles
extract
antimicrobial activity
