摘要
目的探讨肿瘤转移抑制基因(BRMS1)在不同转移性乳腺癌细胞中的表达及其与组蛋白去乙酰化酶(HDAC)活性的关系和意义。方法采用RT-PCR技术检测3种细胞株〔非转移性MCF-7(A株),低转移性MDA-MB-453(B株),高转移性MDA-MB-231(C株)〕中BRMS1基因的表达。用Western blot检测在上述3种细胞株中BRMS1蛋白的表达。ELISA检测3种细胞株中HDAC的活性。结果(1)在A,B,C 3种细胞株中BRMS1基因表达依次比例为3.1∶2.0∶1.0。C细胞株比A细胞株BRMS1基因表达降低2.1倍,BRMS1基因表达随转移程度的增高而明显降低(P<0.001)。(2)在A,B,C 3种细胞株中BRMS1蛋白表达依次比例为3.2∶1.9∶1.0。C细胞株比A细胞株BRMS1蛋白表达降低2.2倍,BRMS1随转移程度的增高而明显降低(P<0.001)。(3)A,B,C 3种细胞株中HDAC活性依次比例为1.0∶1.8∶2.6。C细胞株比A细胞株HDAC活性增高1.6倍,随转移程度的增高HDAC活性升高(P<0.001)。结论(1)BRMS1基因和蛋白的表达在3种细胞株中随转移程度的增高而呈递减趋势。(2)HDAC活性在3种细胞株中随转移潜能的增高而呈递增趋势;提示BRMS1可能通过参与调节HDAC活性调控乳腺癌的转移。
Objective To investigate the expression of BRMS1 in different metastatic breast cancer ceils and its relation to HDAC activity. Methods The gene expression of BRMS 1 in 3 types breast cancer cell lines [ non-metastasis MCF-7 （ A cell line ） , low metastasis MDA-MB-453 （ B cell line ） and high metastasis MDA-MB-231 （C cell line ）~ was determied by RT-PCR technique ; the protein expression of BRMS1 was measured with Western blot technique and HDAC activity by enzyme linked immunosorbent assay. Results （ 1 ） Gene expressive ratio of BRMS 1 was 3. 1 ： 2.0 ： 1.0 in the A, B, C cell lines, respectively. The gene expression of BRMS1 in the C cell line decreased 210% compared to A cell line, and expression of BRMS1 was markedly reduced as the degree of metastasis increased （ P 〈 0.001 ）. （ 2 ） protein expression ratio of BRMS1 was 3.2： 1.9：1.0 in the A,B ,C cell lines. The protein expression of BRMS1 in the C cell line decreased by 220% compared to A cell line, and protein expressive of BRMS1 was reduced in relation to metastasis degree of breast cancer cell line （ P 〈 0. 001 ）. （ 3 ） HDAC activity ratio was 1.0： 1. 8：2.6 in the A,B,C cell lines . HDAC activity in the C cell line increased 160% compared to A cell line, HDAC activity increased in relation to metastasis degree of the cell line （ P 〈 0.001 ）. Conclusions （ 1 ） Gene and protein expression of BRMS1 is reduced according to the metastasis degree increasing of the cell line. （2） HDAC activity increases with increase of degree of metastasis of the 3 cell types, and suggests that BRMS1 may regulate breast cancer metastasis via HDAC activity.
出处
《中国普通外科杂志》
CAS
CSCD
2008年第5期458-461,共4页
China Journal of General Surgery
