摘要
目的探讨白介素-4(interleukin-4,IL-4),白介素-6(interleukin-6,IL-6)和调节激活正常T细胞表达的分泌因子(regulated on activation normal T-cell expressed and secreted,RANTES)在鼻息肉中的表达及在其发病中的作用。方法对30例鼻息肉组织和20例鼻中隔偏曲患者下鼻甲黏膜组织行苏木素-伊红(hematoxy-lin-eosin,HE)染色,并应用免疫组织化学二步法检测两种组织内IL-4,IL-6和RANTES的表达情况。结果(1)鼻息肉组织中嗜酸性粒细胞含量较多,且明显高于下鼻甲组织(χ2=17.014,P<0.01),而嗜中性粒细胞含量在两组间差异无统计学意义;(2)鼻息肉组织中IL-4和RANTES阳性细胞数明显高于下鼻甲组织(χ2=24.956,χ2=22.121,P<0.01),而IL-6在两组间差异无统计学意义;(3)IL-4和RANTES在鼻息肉组织中的表达呈明显正相关(r=0.843,P<0.01),而IL-4和IL-6则无相关性。结论炎性细胞因子IL-4和RANTES在鼻息肉的发病中起一定作用,前者作用于后者可能通过促进嗜酸性粒细胞的生成,从而导致鼻息肉的形成,而嗜中性粒细胞及IL-6对鼻息肉的发病未发现有明显的作用。
Objective To investigate the expression of interleukin-4 （IL-4）, IL-6 and regulated on activation normal T-cell expressed and secreted（RANTES） in nasal polyps and their role in the pathogenesis of nasal polyps. Methods Thirty nasal polyps （NP） and 20 inferior turbinates （IT） mucosal from patients with deviated nasal septum were prepared for hematoxylin-eosin （HE） staining. Immunohistochemistry （two-step method） was used to detect the expression of IL-4,IL-6 and RANTES in the above tissues. Results （ 1 ） The amount of eosinophils in NP was higher than that in IT （X^2 = 17.014, P 〈 0.01 ）. No difference in the amout of neutrophils was seen between NP and IT; （2）The IL-4 and RANTES positive cells in NP were significantly more than those in IT （x^2 =24.956,x^2 =22. 121, P 〈0.01）, while no difference in the IL-6 positive expression was seen between NP and IT; （3）A positive correlation was observed between IL-4 and RANTES expression in NP （r =0. 843, P 〈0.01 ）, but not between IL-4 and IL-6 expression. Conclu- sions The interaction of IL-4 ,RANTES may play an important role in the pathogenesis of nasal polyps by prompting the adhesion and migration of eosinophils but not the neutrophils and IL-6. （ Chin J Ophthalmol and Otorhinolaryngol,2008, 8 ： 148-150）
出处
《中国眼耳鼻喉科杂志》
2008年第3期148-150,I0001,共4页
Chinese Journal of Ophthalmology and Otorhinolaryngology
