摘要
目的研究局部注射重组人类可溶性受体对大鼠正畸牙移动进程的影响。方法选取64只雄性SD大鼠,从加力第1天起在大鼠牙齿局部分别注射重组人类可溶性白细胞介素-1受体Ⅱ(sIL-1-RⅡ)、可溶性肿瘤坏死因子受体Ⅰ(sTNF-RⅠ)、两者混合物,测量磨牙移动距离,用苏木精-伊红(HE)染色观察被移动磨牙牙周组织形态学变化,并应用抗酒石酸盐酸性磷酸酶(TRAP)组织化学染色对破骨细胞和破牙骨质细胞的数量及分布的时相性改变进行分析。结果各受体组大鼠磨牙牙周组织形态学时相性变化与对照组相似,但各时间点牙槽骨吸收程度均较轻微,多数大鼠牙根表面很少或几乎没有明显牙骨质吸收迹象。与对照组相比,在第14天所有受体注射组牙槽骨及牙根表面TRAP染色阳性细胞降低了约50%(P<0.05),但受体组组间差异无统计学意义(P>0.05)。结论在大鼠正畸牙局部注射重组人类可溶性白细胞介素-1和肿瘤坏死因子受体,可以减少牙齿移动距离并减少牙根吸收。
Objective To investigate the effects of local injections of recombinant soluble human receptors on experimental orthodontic tooth movement in the rat. Methods 64 male Spragne-Dawley rats were observed. Starting at day 1, three groups of animals each received local injections of soluble interleukin-1 receptor II （sIL-1-R Ⅱ ）, soluble tumor necrosis factor receptor Ⅰ （sTNF-R Ⅰ ） and their combinations. One group served as the control. The amount of tooth movement was recorded and selected tissue sections were stained with hematoxylineosin （HE） to observe the histological morphologic alterations of the periodontal tissues and also were stained with tartrate-resistant acid phosphatase （TRAP） histochemistry to analyze the changes of the amount and distribution of osteoclasts and odontoclasts. Results The time-depended histomorphology changes in each receptor group were similar to those in the control group, but the resorption of alveolar bone was slighter at each time point and the surface of root appeared no or a few cement resorption signs. On day 14, the TRAP-positive ceils on the surface of alveolar bone and root were reduced by approximately 50% relative to those in the control group （P〈0.05）. Whereas statistical tests revealed there were no significant differences among the experimental groups （P〉0.05）. Conclusion Local injections of recombinant human sIL-1-R Ⅱ and sTNF-R Ⅰ in the orthodontic tooth of rats could reduce the amount and velocity of orthodontic tooth movement and reduce the incidence of root resorption.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第2期189-193,共5页
West China Journal of Stomatology
基金
上海市科学技术委员会科研计划资助项目(05ZR14128)
