摘要
以外源基因(RDV MP-)和玉米内源基因Zein作为PCR扩增对象,旨在建立一种简便有效的转基因玉米及其产品的二重PCR检测技术。转外源基因(RDV MP-)材料的常规PCR检测结果证明外源基因在转基因材料中可稳定遗传;对常规PCR检测的阴性结果材料进行二重PCR检测,扩增结果除进一步证实常规PCR检测的阴性结果结论外,还证明提取的植物总DNA质量符合试验要求,进而从二重PCR检测结果还得出常规PCR检测的阴性结果出错率为1.4%。此方法简单,实用,结果可靠,可适用于转基因植物及产品的检测。
A simple and effective method for the duplex PCR detection was developed by using sequences of exogenous gene （ RDV MP- ） and endogenous gene （Zein） as templates for PCR amplification. The results of routine PCR amplification for RDV MP- gene in transgenic maize suggested that RDV MP- gene can stably inheritate in transgenic plants and their progenies; The duplex PCR detection of all negative and part positive samples that obtained by routine PCR amplification confirmed that above negative results were exact, also showed that the quality of extracted DNA can meet the need of PCR amplification. The error ratio of negative samples was 1.4%. The method used in this study was simple and credible and can be used to detect transgenic plants and their oroducts.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第5期83-86,共4页
China Biotechnology
基金
国家"863"计划资助项目(2001AA212131)
