摘要
建立参芪片中黄芪甲苷的含量测定方法。采用HPLC—ELSD法，色谱柱为大连依利特Hypersil—C18；流动相为乙腈-水（32：68）；流速1．0mL·min^-1；漂移管温度为80℃。黄芪甲苷进样量在0．7452-7．452μg范围内，积分值的对数与进样量的对数线性关系良好，r=0．9985，平均回收率97．36%，RSD为2．32％。本方法无干扰，准确、重现性良好，可作为含量控制方法。
To establish a method to determine astragaloside in Shenqi Tablets. HPLC--ELSD was used to determine directly astragaloside on Hypersil C18 column. Using acetonitrile： water （32 ： 68） as mobile phase , the flow rate was 1.0 mL · min^- 1. The temperature of drift tube for ELSD was 80 ℃. r= 0. 9985. Good linear relation of logA with logW was achieved when the sample size of astragaloside ranged at 0. 7452-7. 452μg. The average recovery of astragaloside was 97.36% , RSD was 2. 32%. The method is simple, accurate and reproducible. It can be used as the quality control method of shenqi Tablet.
出处
《中国药事》
CAS
2008年第5期411-412,共2页
Chinese Pharmaceutical Affairs
关键词
参芪片
HPLC
黄芪甲苷
含量测定
Shenqi Tablets
HPLC--ELSD
astragaloside
determination
