摘要
建立测定人血浆中对乙酰氨基酚浓度的HPI．C法，并应用于人体药代动力学研究。血浆样品用6％高氯酸沉淀蛋白。色谱柱为Zorbax Eclipse XDB-C18柱，流动相为0．02mol·L^-1甲酸铵溶液（含甲酸0．2％）-甲醇-乙腈（88：6：6），流速为1．0mL·min^-1，紫外检测波长245nm。血浆中内源性物质对样品测定无干扰。本方法线性范围为0．1-20μg·mL^-1（r=0．9996），最低定量浓度为0．1μg·mL^-1，方法回收率为99．0％-100．2％，日内、日间RSD均小于6％。本法简便、准确，适用于对乙酰氨基酚药代动力学的研究。
A HPLC method was established for the determination of acetaminophen in human plasma and for human pharmacokinetics study. Proteins in plasma samples were precipitated by 6% perchloric acid. A Zorbax Eclipse XDB-C18 was used with the mobile phase of 0.02 mol · L^-1 ammonium formate solution （contained 0. 2% formic acid） -methanol-acetonitrile （88 ： 6 ： 6） at a flow rate of 1.0 mL · min^-1. The detection wavelength was at 245nm. The endogenous substance in plasma did not interfere with the determination of the samples. The linear range was 0.1 -20μg · mL^-1 （r=0. 9996）, and the limit of quantitation was 0.1μg · mL^-1. The method recovery was 99.0%- 100.2%. Both within-day RSD and between-day RSD were less than 6%. The method is simple and accurate. It is suitable for the pharmacokinetic study of acetaminophen.
出处
《中国药事》
CAS
2008年第5期402-404,共3页
Chinese Pharmaceutical Affairs
