摘要
本文采用３’端特异性ＰＣＲ技术对３６例慢性乙型肝炎患者进行了ＨＢＶ前Ｃ区１８９６位点突变检测。结果发现４例ＨＢＶ突变株感染，３例为混合感染，１例为单纯突变株感染。作者对使用３’端特异性ＰＣＲ对ＨＢＶ突变株检测的作用及ＨＢＶ突变株在乙肝病情发生、发展中的作用进行了探讨。
The 3mutation specific Polymerase Chain Reaction technique was used to detect HBV pre C mutation in 36 cases of chronic hepatitis B.Four patients were detected to have HBV DNA pre C mutation.The total detection rate is 11.1%.The detection rate in chronic active hepatitis and chronic persistence hepatitis is 3/19(15.7%)、1/12(8.3%)respectively.Our result shows that there are pre C mutation patients in Foshan district.The significance of pre C mutation in the pathogenesis and development of the disease still need investigation.The 3specific PCR is a useful technique for research in the field of HBV mutation in small hospitals like ours.
出处
《临床肝胆病杂志》
CAS
北大核心
1997年第4期204-205,共2页
Journal of Clinical Hepatology
