摘要
运用错配聚合酶链反应 (PCR)和限制性片段长度多态性 (RFLP)分析方法 ,检测乙型肝炎病毒(HBV)基因前C区信号酶裂解位点 (T186 2 )变异在重型乙型肝炎、乙型肝炎无症状携带者 (AsC)、乙型肝炎病毒感染后肝硬化 (LC)及慢性肝炎 (CH)病人中的发生率 ,以探讨T186 2变异在各组肝病中的临床意义。T186 2变异在 4组病人中的发生率分别为 :重型乙型肝炎 17.3% (9/ 5 2 ) ,AsC无一例变异 ,LC 2 .7% (1/ 37) ,CH 2 .3% (1/44 )。重型乙型肝炎病人T186 2变异率与AsC、LC和CH比较 ,差异有显著性意义 (P <0 .0 1) ,而AsC与LC、CH比较 ,T186 2变异差异无显著意义 (P >0 .0 5 )。提示T186 2变异与乙型肝炎病毒引起的急性重症肝炎或慢性肝炎急性加重有关。
A simple method of mismatch PCR-RFLP was established to detect HBV pre-C signal enzyme cleavage site mutation (T1862) in 52 patients with severe hepatitis B, 40 patients with AsC, 37 patients with LC and 44 patients with CH. The results were proved by direct sequencing. The prevalence of T1862 mutation rate was 17.3% (9/52) in patients with severe hepatitis B,0 in patients with AsC,2 7%(1/37) in pafients with LC,and 2 3%(1/44) in pafients with CH. Significant difference was found in mutation rate between severe hepatitis B group and the rest three groups (P<0.01), and no significant difference was found in mutation rate among the rest three groups(P>0.05), suggesting that T1862 mutation is highly corelative to severe hepatitis B or acute exacerbation of chronic hepatitis.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2001年第3期198-200,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助课题! (编号 3 963 0 2 0 80 )
军队医药卫生重点资助课题! (编号 96Z0 2 4)
