摘要
目的探讨膀胱移行细胞癌(BTCC)突变型P53基因(mP53)和增殖细胞核抗原(PCNA)表达的相关性及临床意义。方法应用免疫组化二步法,检测50例BTCC组织和10例正常膀胱黏膜中mP53、PCNA的表达,结合临床病理资料进行统计分析。结果50例BTCC mP53阳性表达率66%,PCNA阳性表达率76%;10例正常膀胱黏膜mP53和PCNA的表达均为阴性(P<0.05);mP53和PCNA表达呈正相关(r=0.63,P<0.05);mP53和PCNA阳性表达率在浸润型肿瘤组、G2和G3级肿瘤组、复发组均较高(P均<0.05)。结论mP53和PCNA在膀胱移行细胞癌中表达均增高,二者与BTCC分期、细胞的分级、复发相关,可作为判断BTCC预后的参考指标。
Objective To study the relationship between the expression of mutant P53 （mP53） and proliferating cell nuclear antigen （PCNA） and their clinical significances in transitional cell carcinoma of the bladder. Methods Immunohistochemistry was used to detect the expression of mutant P53 protein and PCNA in 10 tissues of normal mucosa of bladder and 50 tissues of primary transitional cell carcinoma of the bladder （BTCC）. The results were analysed with the clinical pathologic characters of BTCC. Results In the 50 tissues of BTCC, the positive expression rate of mutant P53 and PCNA was 66% and 76%. In the 10 tissues of normal mucosa of bladder, the expressions of mutant P53 protein and PCNA were negative （P〈0.05）. There was positive correlation between the expression of mutant P53 and PCNA （r = 0.63, P〈0.01）. In the group of invasive stage, high grade（G2＋ G3） or recurrence, the positive expression rate of mutant P53 or PCNA was higher （P〈0.05）. Conclusion The expression of mutant P53 has positive correlation with the expression of PCNA. The expression of mutant P53 or PCNA has positive correlation with different stages, grades and recurrence; therefore, they have prognostic value.
出处
《现代泌尿外科杂志》
CAS
2008年第3期179-181,共3页
Journal of Modern Urology
关键词
膀胱移行细胞癌
突变型P53基因
增殖细胞核抗原
免疫组化
transitional cell carcinoma of the bladder
mutant P53
proliferating cell nuclear antigen
immunohistochemistry
