摘要
目的探讨肠球菌表面蛋白基因(Esp基因)的表达与肠球菌致病性的相互关系。方法采用PCR检测自我院住院或门诊患儿分离的152株致病性肠球菌Esp基因。结果152株肠球菌中98株检出Esp基因,占64.5%;30株粪便分离的非致病性肠球菌均未检出Esp基因(P<0.01);粪肠球菌、屎肠球菌Esp基因检测阳性率分别为90.9%和28.1%,粪肠球菌明显高于屎肠球菌(P<0.01);152株致病肠球菌来自不同的感染部位,尿液、阴道分泌物、痰液、血液和脐分泌物标本Esp基因的阳性率分别为83.7%、55.6%、50.0%、43.0%和33.0%,住院患儿标本中分离的致病性肠球菌的Esp基因阳性率为75.0%,明显高于门诊患儿阳性率37.5%(P<0.001)。结论肠球菌的Esp基因在肠球菌对宿主细胞的黏附定植以及逃避宿主免疫清除方面起到重要的作用。
Objective To study the relationship between Esp gene and the pathogenesis of enterococcal isolates. Methods Polymerase chain reaction was used to test the Esp gene. Results Of the 152 enterococcal strains isolated from children, 98 were positive for Esp gene. Esp gene was not identified in the 30 strains isolated from stool （P〈0. 001）. The prevalence of Esp gene in E. faecalis and E. faecium was 90.9% and 28.1% respectively. The prevalence in E. faecalis was significantly higher than in E. faecium （P〈0. 001）. The prevalence Esp gene in urine, umbilical swab, genital swab, blood and sputum was 83. 7%, 55.6%, 50.0%, 43.0% and 33.0%, respectively. The prevalence of Esp gene was 75.0% in inpatients and 37.5% in outpatients （P〈0. 001）. Conclusions Esp gene in Enterococcus plays an important role in adherence and colonization, as well as escaping immune elimination.
出处
《中国感染与化疗杂志》
CAS
2008年第3期222-224,共3页
Chinese Journal of Infection and Chemotherapy
基金
国家科技部十五攻关课题基金资助(2004BA720A09-01)
