摘要
目的建立稳定表达红色或绿色荧光的人肝癌裸鼠转移模型。方法用带红色或绿色荧光蛋白基因的假慢病毒感染人高转移潜能肝癌细胞HCCLM3，获得稳定表达红色或绿色荧光的新细胞系HCCLM3-R和HCCLM3-G。1×10^7细胞皮下接种、2mm^3组织块原位移植裸鼠，建立稳定表达荧光的人肝癌裸鼠转移模型。结果皮下接种5只裸鼠和肝脏原位移植10只裸鼠肿瘤全部生长，经180d裸鼠体内连续6次传代肿瘤荧光表达稳定，肝内播散、肺转移和腹腔转移检出率分别为100％、100％和90％。结论采用HCCLM3-R、HCCLM3-G细胞所建立的荧光表达人肝癌裸鼠转移模型，是一个较理想的研究肝癌生长和转移的动物模型。
Objective To establish stable RFP- or GFP-expressing HCC xenograft model in nude mice. Methods HCCLM3, a human HCC cell line with high metastatic potential was transfected with full-length cDNA of RFP or GFP by lentivirus. 1 × 10^7 stable RFP- and GFP-expressing HCC cells namely HCCLM3-R and HCCLM3-G were subcutaneously injected and HCCLM3-R or HCCLM3-G orthotopic model in nude mice was established by surgical implantation of 2 mm^3 subcutaneous tumor tissue pieces. Results One hundred and eighty days after 6 passages in nude mice, HCCLM3-R and HCCLM3-G xenograft model still expressed red and green fluorescence stably and intensely. One hundred percent tumorigenesis was achieved when HCCLM3-R and HCCLM3-G ceils were injected subcutaneously in 5 nude mice or when HCCLM3-R and HCCLM3-G tumor tissue were implanted into the liver in 10 nude mice. The ratio of tumor metastasis to liver,lung and abdomen was 100% ,100% and 90% ,respectively. Conclusion Stable fluorescent protein-expressing HCC xenograft models derived from HCCLM3-R and HCCLM3-G cell lines could be useful for studying the mechanisms of tumor growth and metastasis of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第4期516-518,F0004,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30670953）
