摘要
近年来,肝干细胞的基础研究已取得重大进展,肝干细胞可分化为肝细胞和胆管细胞已经得到了共识。目前多用两步胶原酶灌流消化法、密度梯度离心法、离心淘洗技术、荧光激活细胞筛选法及免疫磁珠细胞筛选法进行肝干细胞的分离纯化。然而肝干细胞的定向分化是一个复杂的过程,是在一些细胞内外因素及肝脏微环境的作用下完成的。肝脏微环境有利于干细胞的生长、分化与功能发挥。肝细胞生长因子能够促进肝干细胞生长增殖,并且促进其向成熟肝细胞分化,肝细胞转录因子在肝干细胞的分化调控中也起重要作用。肝癌可能是肝干细胞分化不全或分化异常所致,肝干细胞可望作为靶向基因治疗肝癌极具潜力的载体细胞。
Present basic researches on hepatic stem cells have obtained great progress. It is believed that hepatic stem cells can differentiate into hepatic cells and biliary duct cells. Hepatic stem cells are isolated and cultured by two-step collagenase digestion method, density gradient centrifugation, centrifugal elutriation, fluorescent activation screening method and immunomagnetic beads screening method. Directional differentiation of hepatic stem cells is a complicated process, which is affected by exterior and interior cell factors under liver microenvironment where are beneficial to growth, differentiation and function of stem cells. Hepatocyte growth factor can promote the proliferation of hepatic stem cells, and accelerate the differentiation into mature hepatic cells. Transcription factor of hepatic cells play a crucial effect in regulating the differentiation of hepatic stem cells. Incomplete or abnormal differentiation of hepatic stem cells can be responsible for liver cancer. Hepatic stem cells are the potential carrier cells as a target gene for liver cancer.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第12期2355-2358,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
