摘要
目的：采用HPLC测定林下山参、园参及红参中3种主要人参皂苷-Rg1、-Re、-Rb1，的含量；采用HPLC建立林下山参药材的指纹图谱。方法：人参皂苷含量测定HPLC条件：色谱柱为DiamonsilC18柱（250mm×4．6mm，5μm），流动相为乙腈、水（梯度洗脱），检测波长203nm，柱温30％，流速1．0mL·min^-1，分析时间100min；指纹图谱HPLC条件：色谱柱为Zorbax SB＋C18柱（150mm×4．6mm，5μm），流动相：乙腈-0．05％磷酸水溶液（梯度洗脱），检测波长203nm，柱温30℃，流速1．0mL·min^-1，分析时间90min。结果：园参、红参和林下山参的人参皂苷Rg1，Rb1）。含量测定值没有显著性差异，-Re的含量测定值有差异，林下山参〉园参〉红参；共标示出林下山参药材指纹图谱中19个共有峰。利用指纹图谱相似度测试软件，以夹角余弦为测度，测得10批林下山参药材指纹图谱相似度结果均在0．90—1．00。结论：人参皂苷含量测定方法稳定准确，重现性好，可作为测定林下山参的3种皂苷的较好方法；本实验所建立的林下山参药材指纹图谱分析方法准确、重复性好，为有效控制林下山参药材的质量提供了可靠依据。
Objective： To investigate the difference of contents of ginsenoside -Rg1, -Re, -Rb1 of Yuanshen, Hongshen and Linxiashanshen by HPLC and to establish fingerprint of Linxiashanshen by HPLC. Methods： Content determination of ginsenosides： HPLC with Diamonsil C18 （250mm ×4. 6mm, 5μm） column was used, the acetonitrile - water （gradient elution： 0 -35min acetonitrile 19, 35 -55min acetonitrile 19 -29 , 55 -70min acetonitrile 29, 70 -100 min acetonitrile 29 -40） as a mobile phase with the column temperature at 30℃ and the flow rate 1.0mL·min^-1. The UV wavelength used for detection was set at 203nm and the analysis time was 100min. Condition of fingerprint ： Separation was performed on an Agilent Zorbax SB-C18 （ 150mm ×4. 6mm, 5μm） analytical column with mobile phase consisting of acetonitrile and 0. 05% H3PO4 （gradient elution ： 0 - 15min acetonitrile 19, 15 - 30min acetonitrile 19 - 29, 30 - domin acetonitrile 29, 40 - 50min acetonitrile 29 -35, 50 -56min acetonitrile 35 -45, 56 -80min acetonitrile 45 -90, 80 -90min acetonitrile 90） with the column temperature at 30℃ and the flow rate 1.0mL·min^-1. The UV wavelength used for detection was set at 203nm and the analysis time was 90min. Results： The contents of ginsenoside - Rg1 and - Rb1 are similar in the three kinds of gin- seng. However, -Re is highest in Linxiashanshen, then Yuanshen and Hongshen; 19 co-peaks on the HPLC fingerprints of Linxiashanshen were indicated. The similarities were determined by Cosine. The similarity result of analysis were 0. 90 - 1.00. Conclusion： This method can be good for the content determination of ginsendside-Rgl, -Re and -Rb1 of Linxiashanshen. In addition, suitable fingerprints were obtained which can be used for the quality control of Linxiashanshen.
出处
《中国现代中药》
CAS
2008年第4期12-15,共4页
Modern Chinese Medicine
关键词
人参
林下山参
人参皂苷
HPLC
指纹图谱
Panax gingseng C. A. Mey.
Linxiashanshen
Ginsenoside
HPLC
Chromatographic fingerprint
