摘要
用分光光度法研究了色氨酸与2,3-二氯-5,6-二氰-1,4-苯醌(DDBQ)的荷移反应。由实验得知,在硼砂缓冲溶液中,40℃水浴加热30min,两者可生成稳定的1∶2型荷移络合物。此络合物的λmax=341nm,表观摩尔吸光系数ε=1.4×105L·mol-1·cm-1,浓度在2—12μg/mL的范围内符合比耳定律,回收率在98.73%—101.7%之内,相对标准偏差在3.2%以内。
The charge transfer complex of tryptophan with 2, 3-dichloro-5, 6-dicyano-1, 4- benzoquinone was studied by spectrophotometry in borax buffer solution. The apparent molar absorptivity was determined as 1.4×10^5L·mol^-1·cm^-1 at 341nm,and Beer＇s law was obeyed in the range of 2- 12μg/mL. The relative standard deviation and the recovery are in the range of 1.5-3.2% and 98.73-101.7% ,respectively. This method was successfully applied to the determinauon of tryptophan in sample.
出处
《光谱实验室》
CAS
CSCD
2008年第2期202-204,共3页
Chinese Journal of Spectroscopy Laboratory
