摘要
目的:观察小檗碱(Ber)对舒张性心力衰竭(diastole heart failure,DHF)大鼠血流动力学和心肌细胞内钙离子浓度[Ca2+]i的影响。方法:Wistar大鼠腹主动脉缩窄法建立DHF模型;术后4周,模型大鼠随机分为DHF模型组,Ber高、中、低剂量组(63,42,21mg·kg-1.d-1)4组(n=10),另有假手术组10只。连续给药4周后,应用十六导生理记录仪测定血流动力学指标;用激光扫描共聚焦显微镜(LSCM)扫描用Fluo-3AM负载好的心肌细胞内的荧光强度(FI)来表示[Ca2+]i。结果:模型组与假手术组比,左心室收缩压(LVSP)、左室内压最大上升速率(+dp/dtmax)无显著变化,左心室舒张末期内压(LVEDP)显著升高(P<0.01),左室内压最大下降速率(-dp/dtmax)显著降低(P<0.01),左室松弛时间常数(T)数值显著延长(P<0.01);心肌细胞内[Ca2+]i明显增高(P<0.05)。与模型组比较,Ber高剂量组比中剂量组低剂量组更显著降低LVEDP,显著升高-dp/dtmax(P<0.01),显著缩短T(P<0.01),显著降低心肌细胞内[Ca2+]i(P<0.01)。结论:Ber高剂量组可以明显改善DHF大鼠血流动力学和降低心肌细胞内[Ca2+]i。
Objective： To study the effects of different doses of berberine on hemodynamic parameters and calcium ion concentration （ [ Ca2 ＋ ] i ） of diastole heart failure rat models. Method ： The mouse models of diastole heart failure were made by the imcomplete ligation of abdominal aorta. Forty Wistar heart failure rats were divided randomly into four groups, with 10 for each group （ n= 10）. Heart failure rats were treated according to different doses drugs as follows： Model （natrii chloride 2 mL）, berberine （63 mg· kg^-1· d^- 1 ）, berberine （42 mg · kg^-1· d^-1）, berberine （21 mg ·kg^-1· d^ -1 ） ig, for each of the four groups respectively, 4 weeks after coarctation of ascending aorta operation; and 10 age matched sham operation group was taken as control （natrii chloridi, 2 mL）. After administration four weeks, cardiac function was determined by catheter. Isolate single cardiomyocytes of rat which were loaded with Ca^2＋-sensitive fluorescent indicator Fluo-3/AM. [ Ca^2＋ ]i represented by fluorescent intensity[ FI] was measured by laser scanning cofocal microscope [ LSCM ]. Result： The rats of operation group have no significant changes with those of the control on left ventricular systolic pressure （LVSP） and maximal rising rate of ventricular pressure （ ＋ dp/dtmax）, left ventricular end diastolic pressure （LVEDP） was much higher in operation group （P 〈0. 01 ）, but maximal falling rate of ventricular pressure （-dp/dtmax） was depressed （P 〈0. 01 ） , left ventricular relax time constant quantity （T） was markedly extended （P 〈0. 01 ）. [ Ca^2＋ ]i level in carkiac muscle cell was elevated markedly （ P 〈 0. 05 ）. Compared with operation group, high dose of Ber can decrease LEVDP, improve （ - dp/dtmax） （P 〈0. 01 ）, decurtate left ventricular relax time constant quantity （P 〈0. 01 ） and decrease [ Ca^2＋ ]i level better than those of middle and low dose group （P 〈0. 01 ）. Conclusion： Berberine is an effective new potent drug for conspicuous symptom rehef of heart failure with positive dose dependency and step down [ Ca^2 ＋ ] i of myocardial cell.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第7期818-821,共4页
China Journal of Chinese Materia Medica
