摘要
目的:研究β-榄香烯联合三苯氧胺对乳腺癌MCF-7细胞生长有无协同抑制作用,并探讨其可能的机制。方法:采用MTT法检测β-榄香烯联合三苯氧胺对MCF-7细胞的增殖抑制作用,流式细胞仪测定细胞周期和凋亡率,免疫组化SP法检测bcl-2、pS2表达变化。结果:不同剂量的β-榄香烯联合治疗剂量的三苯氧胺,吸光度均值较单用三苯氧胺明显下降,相应的细胞抑制作用明显上升;流式细胞仪检测G/G期细胞比例增加,用药后凋亡率升高,bcl-2、pS2在联合用药后表达明显下调。结论:β-榄香烯使三苯氧胺对乳腺癌MCF-7细胞的抑制作用显著增强,提示榄香烯与三苯氧胺具有协同增效作用,其作用机制与影响细胞周期进程,协同促进凋亡,下调bcl-2、pS2表达有关。
Objective： To investigate whether MCF -7 cell strain of breast cancer can be restrained by [3 - elemene in coordination with tamoxifen（TAM） , and its possible mechanism. Methods： MTr method was used to test the inhibitory effect of β - elemence combined with TAM on MCF - 7 cell strain, FCM mensurate cell cycle and the percentage of apoptosis, SP method to test bcl - 2, pS2 expression change. Results. Different dosage of β - elemene and curative dose of tamoxifen reduced absorptions significantly compared with the group of tamoxifen alone, meanwhile the inhibitory rates increased significantly. The increaseing of G0/G1 phase cell proportion tested by FCM, the percentage of apoptosis elevated after treated by the medicine, bcl- 2, pS2 express obviously declined. Conclusion： β -elemene enhances the inhibitory effect of tamoxifen on MCF -7 cell strain of breast cancer significantly ,which in- dicates the inhibition of TAM on breast cancer cell strain can be synergized by β - elemene, the mechanism concern with influence cell cycle, promotes apoptosis, and downregnlates bcl -2, pS2 expression.
出处
《现代肿瘤医学》
CAS
2008年第4期510-514,共5页
Journal of Modern Oncology
基金
陕西省自然科学基金资助项目[20021210-G3(05)]
