期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

奶牛乳腺炎金黄色葡萄球菌超抗原的检测 被引量：2

Detection of Superantigens from Staphylococcus aureus causing bovine mastitis
在线阅读 下载PDF
导出
摘要 用PCR方法对分离到的临床型乳腺炎金黄色葡萄球菌124株,隐性乳腺炎金黄色葡萄球菌213株,进行超抗原毒素sea,seb,sec,sed,see和tst基因的检测,结果表明:奶牛临床型乳腺炎金黄色葡萄球菌肠毒素基因和tst基因的阳性率为27.42%,隐性乳腺炎金黄色葡萄球菌肠毒素基因的阳性率为1.41%,奶牛乳腺炎金黄色葡萄球菌产生毒素的类型以SEA,TSST-1和SEC为主,对于肠毒素基因和tst基因PCR阳性的金黄色葡萄球菌同时用ELISA和RPLA两种方法进行检测,3种检测方法结果基本一致。 One hundred and twenty-four Staphylococcus aureus （S. aureus）stratus were isolated from the clinical mastitis milk samples. Two hundreds and thirteen S. aureus isolates caused subclinical mastitis. Enterotoxin genes and tst genes were detected in 337 Staphylococcus aureus isolates using PCR. It was found that 27.42 % of bovine clinical mastitis S. aureus isolates harhoured enterotoxin genes and tst genes, so did 1.41% of bovine subclinical mastitis S. aureus isolates. Enterotoxin A, enterotoxin C and TSST-1 were the major toxins produced by bovine mastitis S. aureus. All S. aureus isolates carrying enterotoxin genes or tst genes were examined by ELISA or RPLA. The results from of the three methods（PCR,ELISA and RPLA） were consistent.
作者 安福生 董玉兰 马保臣
机构地区 中国牧工商(集团)总公司 中国农业大学动物医学院
出处 《中国兽医杂志》 CAS 北大核心 2008年第3期9-11,共3页 Chinese Journal of Veterinary Medicine
关键词 乳腺炎 金黄色葡萄球菌 肠毒素 TSST-1 mastitis S. aureus enterotoxin TSST-1
分类号 S857.2 [农业科学—临床兽医学]
  • 相关文献

参考文献9

  • 1Johnson W M, Tyler S D,Ewan E P, et al. Detection of genes for enterotoxins, exfoliative toxins and toxic shock syndrome toxin-1 in Staphylococcus aureus by the polymerase-chain-rection[J]. Journal of Clinical Microbiology, 1991,29 (3) : 426-430.
  • 2马保臣,秦卓明,蔡玉梅,董玉兰,柴同杰.多重PCR检测奶牛乳腺炎金黄色葡萄球菌、无乳链球菌、停乳链球菌和酵母菌方法的建立与应用[J].畜牧兽医学报,2006,37(11):1202-1208. 被引量：40
  • 3Kenny K, Reiser R F,Bastida-Corcucra F D, et al. Production of enterotoxins and toxin by bovine mammary isolates of Staphylococcus aureus [J]. Journal of Clinical Microbiology, 1993,31 : 706-707.
  • 4Bruckler J, Klima H, Schaeg W, et al. Staphylococcus aureus aus Kuhen mit subklinischen euterinfektionen[J]. Zentralblatt Veterinarmedizin Reihe B, 1981,28:494-499.
  • 5Larsen H D,Aarestrup F M,Jensen N E. Geographical variation in the presence of genes encoding superantigenic exotoxins and β-hemolysin among Straphylococcus aureus isolated from bovine mastitis in Europe and USA[J]. Veterinary Microbiology, 2002,85 : 61-67.
  • 6Zschock M,Botzler D, Blocher S, et al. Detection of genes for enterotoxins(ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase chain reaction[J]. International Dairy Journal, 2000, 10 : 569-574.
  • 7Suk kyung LIM, Yi seok JOO, Jin san MOON, et al. Molecular typing of Enterotoxigenic Staphylococcus aureus isolated from bovine mastitis in Korea[J]. J Vet Med Sci, 2004,66: 581-584.
  • 8Takeuchi K, Ishiguro K, Ikegami M, et al. Production of toxic shock syndrome toxin by S. aureus isolated from mastitis cow's milk and farm bulk milk[J]. Veterinary Microbiology, 1998,59:251-258.
  • 9Adekeye D. Enterotoxin production by strains of Staphylococcus aureus isolated from animals and man in Nigeria[J]. Veterinary Microbiology, 1980,5: 143-150.

二级参考文献14

  • 1马保臣,李建基,王春璈.奶牛急性乳腺炎病原菌的分离与鉴定[J].中国兽医杂志,2004,40(11):22-24. 被引量：19
  • 2曹澍泽.兽医微生物学及免疫学技术[M].北京：北京农业大学出版社,1991.120-158.
  • 3Lenoard J, Lascdea S R, Dryyia D. Quanlitation of bacteria in cerebrospinal fluid and blood of children and its diagnostic significance[J]. J Clin Microbiol,1984,19(2):187.
  • 4Teng k, Li M, Yu W, etal. Comparison of PCR with culture for detection of ureaplasma urealylicum in clinical samples from patients with urogeneited infections[J]. J Clin Microbiol, 1994, 32(9): 2 232.
  • 5Phuektes P, Mansell P D, Browning G F. Mutiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and Streptococcal causes of bovine mastitis[J]. J Dairy Sci, 2001, 84:1 140-1 148.
  • 6Skladny H, Buchheidt D, Baust C, etal. Specific detection of aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR[J]. J Clin Microbiol, 1999,37(12):3 865-3 871.
  • 7Amann R I, Ludwig W, Schleifer K H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation [J]. Microbiol Rev,1995, 59(1):143-169.
  • 8Gurtler V, Stanisich V A. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region[J].Microbiology, 1996, 142(Pt1):3-16.
  • 9Jensen M A, Webster J A, Straus N. Rapid identification of bacteria on the basis of polymerase chain reaction analysis[J].Appl Environ Microbiol, 1993, 3: 186-194.
  • 10Leblond-Bourget N, Philippe H, Mangin I, etal. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny[J].Int J Syst Bacteriol,1996, 46: 102-111.

共引文献39

同被引文献18

  • 1富艳玲,武瑞.中药防治奶牛乳房炎的研究进展[J].中兽医医药杂志,2005,24(6):24-25. 被引量：10
  • 2黄宇翔,黄鑫华,李尊江.奶牛乳房炎诊断方法研究进展[J].中国乳业,2005(10):38-40. 被引量：4
  • 3张善瑞,王长法,高运东,仲跻峰,赵宏坤.奶牛乳房炎疫苗的研究进展[J].中国奶牛,2006(8):31-32. 被引量：3
  • 4夏胜超,吴聪明,王绍琛,刘金凤,沈建忠.奶牛乳房炎病例中金黄色葡萄球菌毒素基因的检测[J].中国兽医杂志,2007,43(4):23-24. 被引量：12
  • 5Kerro - Dego O., Prysliak T. , Potter A A.. DNA - protein Immunization against the GapB and GapC Proteins of a Mastitis Isolate of Staphylococci aureus [ J ]. Veterinary Immunology And Immunopathology, 2006,113 : 125 - 138.
  • 6Talbot Brian G. , Lacasse Pierre. Progress in the Development of Mastitis, Vaccines[J]. Livestock Production Science, 2005,98 : 101 - 113.
  • 7Bengtsson B., Unnerstad H., et al. Prevalence and antimicrobial susceptibility of bacteria causing acute clinical mastitis in dairy cows in Sweden 2002- 03 [J ]. Mastitis in Dairy Production, 2005,888 - 990.
  • 8Taponen S. , et at. Clinical characteristics and persistence of bovine mastiffs causedby different species of coagulase- negative staphylococci identified with API or AFLP[J]. Vet Microbiol, 2006, 115: 199 207.
  • 9Taponen S., Kcort J., et al. Bovine intramammary infections caused by coagulase- negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism - based analysis [J]. Dairy Sci,2007,90,3301 -3307.
  • 10Contreras AA.. Mastitis in small ruminants [J]. Small Ruminant Research,2007, (68) : 145-- 153.

引证文献2

二级引证文献3

中国兽医杂志
2008年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部