摘要
目的：了解环一磷酸腺苷（ｃＡＭＰ）、环一磷酸鸟苷（ｃＧＭＰ）含量的变化与脑缺血、再灌注损伤和功能恢复间的关系。方法：以大鼠四血管阻断为模型，采用放免法测定不同状态下血浆和皮层、海马、纹状体中ｃＡＭＰ、ｃＧＭＰ的含量。结果：ｃＡＭＰ在各脑区和血浆中的含量，缺血组、再灌组、腺苷和Ｎ６环乙烷基腺苷（ＣＨＡ）给药组分别与对照组相比均无显著性变化（Ｐ＞００５）。ｃＧＭＰ的血浆含量由对照组的５３２±１３５ｐｍｏｌ／ｍｌ增高至再灌组的１８４６±４１７ｐｍｏｌ／ｍｌ（Ｐ＜００１）。给药３、４组也分别增高至２０１５±５６２ｐｍｏｌ／ｍｌ和２２６５±８３４ｐｍｏｌ／ｍｌ，（Ｐ＜００１），但与再灌组相比无显著性差异（Ｐ＞００５）。其余血浆各组及脑区各组ｃＧＭＰ含量均无显著性变化。结论：各脑区内ｃＡＭＰ和ｃＧＭＰ含量与大鼠脑缺血损伤和恢复无直接关系。血浆内ｃＡＭＰ含量无显著性变化，ｃＧＭＰ含量虽有变化，但作为临床观测指标证据不足。
Objective:To look into the relationship between concentrations of cAMP and cGMP and cerebral ischemia,reperfusion damage and the recovery by adenosine treatment.Methods:Four vessel occlusion model of forebrain ischemia in rat was used. Since cerebral cortex、hippocampus and striatum are the most vulnerable areas to ischemic insult,the concentration of cAMP、cGMP in these areas and plasma were determined by RIA method. Results:The concentration of cAMP in either plasma or different cerebral areas was not changed neither in ischemic group、reperfusion group nor in treatment group with adenosine and N 6 cyclohexyladenosine(CHA),an adenosine receptor agonist,compared with that of control group. The concentration of cGMP in plasma increased from 5 32±1 35 pmol/ml in control group to 18 46±4 17 pmol/ml in reperfusion group,( P <0 01 vs CONT). In treatment 3 and 4 groups it also increased to 20 15±5 62 pmol/ml and 22 65±8 34 pmol/ml,respectively,( P <0 01 vs CONT).The concentration of cGMP of other groups and all the groups in different cerebral areas was not changed.Conclusions:The concentration of cAMP and cGMP in different cerebral areas has no direct relationship with the damage of cerebral ischemia and resuscitation.Although the concentrations of cGMP in plasma were changed,there are not enough evidence to use it as a clinical index.
出处
《中日友好医院学报》
1997年第3期189-192,共4页
Journal of China-Japan Friendship Hospital
基金
卫生部"八.五"攻关基金
关键词
大鼠
脑缺血
再灌注
腺苷
CAMP
CGMP
rats
cerebral ischemia
reperfusion
adnosine cyclic monophosphate(cAMP)
guanosine cyclic monophosphate(cGMP)
adenosine
