摘要
我们在转基因水稻后代中发现了两个生殖器官发育相关的隐性突变体,暂命名为function of reproductive organ1（fro1）和pistilloid-stamen（ps-2）。fro1突变体内外稃闭合,抽穗但不开花;4轮器官结构及数目近正常,但内外稃片抱合扭曲成辣椒状,雌雄蕊发育不完全,无花粉形成,雌蕊不育。ps-2突变体类似Luo等（2006）报道的ps突变体：颖花开裂,内外颖片变窄、变硬,外颖弯曲,但ps只有1-5枚雄蕊转化成雌蕊,而ps-2有5-6枚雄蕊转化成雌蕊,仅有极少数突变颖花留有1枚未转变的雄蕊,突变体雌蕊状结构10个以上,不能结实。通过对突变体fro1和ps-2分别与野生型明恢86正反杂交,与R527、93-11和中花16的杂交后代表型分离规律分析表明：突变体fro1和ps-2是分别由一对隐性基因控制的突变体,其中fro1的F2后代表型分离比均符合3：1,大部分ps-2的F2后代表型分离比偏离3：1。进一步分析fro1和ps-2双突变体的遗传和表型发现：fro1和ps-2基因不等位,是两个独立遗传的基因。植株生活力受纯合ps-2基因的影响,但不受纯合fro1基因的影响。双突变体的内外稃片结构及开裂特征似ps-2,而内外稃包合状与fro1近似,雄蕊仍表现雌化,但数目明显减少。fro1和ps-2双突变体的表型特征暗示了ps-2和fro1间存在一定的互作。
Two rice reproductive organ mutants, which are temporarily named function of reproductive organ 1 （f-ro l） and pistilloid-stamen-2 （ps-2）, respectively, were found in transgenic rice progeny. Mutantfrol had heading but not flowering. Floret shape of fro1 mutant is similar to that of tiny green capsicum, and in which stamens and pistils are abnormal, non-pollen, female sterile. The ps-2 mutant showed that lemma and palea were degenerat- ed, and became narrow and hard like rice ps mutant reported by Luo et al. in 2006. But 5-6 stamens had been transformed into pistilloid-stamen, and more than 10 pistilloid structures in the ps-2 mutants. Genetic analysis of the F1 and F2 populations, which are from the crossesfrol andps-2 mutants and rice varieties Minghui 86, R527, 93-11 and Zhonghual6, respectively, indicated that fro1 and ps-2 mutants were all controlled by single recessive gene. A goodness of fit to 3：1 for frollines and abnormal segregation ratios for part lines ofps-2 were observed in F2 lines. The subsequent analysis of a dual-mutant from the crossed progeny of Frolfrol/PS-2ps-2 revealed that frol and ps-2 were not allele genes and vigor of plant was affected by homologous ps-2, but not homolo- gousfrol. In addition, floral structure of the dual mutants was like that ofps-2 mutant, but they were similar to fro1 mutant in shape. The phenotypic characteristics hinted that gene fro1 and ps-2 might have an interaction each other.
出处
《分子植物育种》
CAS
CSCD
2008年第2期233-238,共6页
Molecular Plant Breeding
基金
福建省自然科学基金项目(B0320002
2007J0056)资助
