摘要
以绿原酸为标准品，研究了Folin-Ciocalteu比色法测定苦丁茶中多酚含量的适宜条件。结果表明，苦丁茶提取液在Folin-Ciocalteu试剂3．0ml、饱和Na2CO3溶液4．5ml、反应温度30℃、反应时间30min的条件下，测定其747nm处的吸光值，多酚浓度在20．0～100．0mg／L范围内与吸光值呈良好的线性关系；稳定性、精密度、重现性和回收率实验的相对标准偏差为0．887％～1．416％；与酒石酸亚铁比色法的测定结果无显著差异。该法是一种简便、快速、准确测定苦丁茶多酚含量的可靠方法。
A quantitative method for the determination of the total content of polyphenols in kudincha was studied by using FC method （Folin-Ciocalteu colorimetry ） with chlorogenic acid as standard. The results showed that the total polyphenol contents of kudincha could be well calculated according to their colorimetric absorption at 747 nm by applying Folin-Ciocalteu reagent 3.0 ml and saturated Na2CO3 4.5 ml at 30℃ for 30 min, and the linear range of standard curve was 20.0-100.0 mg/L. Stability, precision, repetition and recovery rate were in the range of 0.887%-1.416% by RSD （relative standard deviation）. There are not any differences for the results obtained by FC method and ferrous tartrate colourimetry. Therefore, FC method is convenient, fast, and accurate to determine the total polyphenols of kudincha.
出处
《茶叶科学》
CAS
CSCD
北大核心
2008年第2期101-106,共6页
Journal of Tea Science
基金
国家高技术研究发展计划(2007AA10Z351)
南京农业大学高层次人才引进基金(804066)
