摘要
采用丙酮粉抽提，DEAE-SephadexA-50、SephacrylS-300、MonoQ柱层析，从银杏（GinkgobilobaL．）花粉中分高纯化出微管蛋白（tubulin），其两个亚基（α、β）的分子量分别为54kD和52kD．纯化的微管蛋白可与鸡脑微管蛋白抗体发生免疫交叉反应．
Chnkg lelde is a ooical gyYnnOSPe and occupies an important P0Stbo in the evo-lution 0f plant kingd0m. Tubulin was purified from ginkg POlen by acetone preciPitatio, fol-lDwd by DEAE-SePhadex A5O chr0rnatograPhy, SePhaCry S3OO gel filtration, and M0nO Q co-uxnn chrOrnatogrphy. The purified tubulin is electmphoretically hanOgeneO and is cornPOSed 0ftwo shubunits. The aPParen molecthar wewhts of the two subunits are ahaut 54 and 52 ari re-sPectivey, estirnated han the StoPAG. The tubulin fiom ginkgo POllen was idenified by..Western blotting- The cross-reactivity 0f antibo boed against chicken brain tubulin with thepurified POllen tubulin SUggested the sedlarity of diverse tubulins betwee mp and rnammals.
基金
国家自然科学基金!39130060
欧共体资助!CI1-CT93-0341
关键词
银杏
微管蛋白
纯化鉴定
Ginkgo biloba, Tubulin, Purification, Identification
