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结直肠癌IL-10和IL-12的检测及意义 被引量：2

The examination and clinical significance of IL-10 and IL-12 in colorectal carcinoma
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摘要 目的检测IL-10和IL-12在结直肠癌组织中含量及术前和术后3周血清浓度并探讨其意义。方法采用ELISA法检测了46例结直肠癌IL-10和IL-12在癌组织和癌周组织中含量及术前和术后3周血清浓度,与24例良性结肠息肉患者资料为对照。结果结直肠癌组织IL-10含量高于癌周组织,而癌组织IL-12含量低于癌周组织;Ⅲ、Ⅳ期患者癌组织和癌周组织中IL-10含量高于Ⅰ、Ⅱ期,而Ⅰ、Ⅱ期癌组织和癌周组织中IL-12含量高于Ⅲ、Ⅳ。术前和术后1周血清IL-10升高而IL-12降低,术后3周血清IL-10和IL-12浓度接近对照组。癌组IL-10和IL-12呈明显负相关。结论结直肠癌细胞可分泌IL-10抑制IL-12的产生,进而营造免疫抑制微环境,动态检测IL-10和IL-12可反映结直肠癌病期、判断疗效和估计预后。 Objective To investigate the clinical significance of interleukin-10 （IL-10） and interleukin-12 （IL-12） in colorectal carcinoma. Methods The content of IL-10 and IL-12 were examined in colorectal carcinoma and its adjacent tissues by ELISA assay, and so did the concentration in the serum before operation, 1 and 3 weeks after operation in 46 cases of colorectal carcinoma and 24 cases of benign colonic polyp chosen as control. Results The content of IL-10 in carcinoma tissues was higher significantly than that in adjacent tissues, and contra versus about IL-12. The contents of IL-10 were higher in Ⅲ and Ⅳ stages than that of Ⅰ and Ⅱ stages, and contra versus about IL-12. The concentrations of serum IL-10 decreased before and one week after operation, and contra versus about IL-12. But 3 weeks after operation, the level of IL-10 and IL-12 reached proximally to the level of the control group. There was negative correlation between IL-10 and IL-12 in tumor tissues and serum. Conclusion In patients with colorectal carcinoma, carcinoma cell may secrete IL-10 to suppress IL-12, which therefore creates microenvironment of immune suppression, of IL-10 and IL-12 can reflect eoloreetal carcinoma stage, judge curative effect and estimate prognosis.
作者 刘新纯 姚联波 廖茂湖 张磊
机构地区 广州钢铁企业集团医院普通外科 中山大学附属第二医院普通外科
出处 《结直肠肛门外科》 2008年第1期32-34,共3页 Journal of Colorectal & Anal Surgery
关键词 结直肠癌 IL-10 IL-12 Colorectal carcinoma Interleukin-10 Interleukin-12
分类号 R735.3 [医药卫生—肿瘤]
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参考文献10

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二级参考文献3

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共引文献18

同被引文献12

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结直肠肛门外科
2008年 第1期

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